Bovine Brucella Antigen Test With Qualitative And Preliminary VETERINARY Bovine Range

Application:

The Bovine Brucella Antigen Rapid Test Cassette is a lateral flow immunochromatographic assay for the qualitative detection of Bovine Brucella Antigen (B.Brucella Ag ) in cattles serum, plasma, or whole blood .

Description:

The Bovine Brucella Antigen Rapid Test is based on sandwich lateral flow immunochromatographic assay. The test cassette has a testing window for the observation of assay running and result reading. The testing window has an invisible T (test) zone and a C (control) zone before running the assay. When the treated sample was applied into the sample well on the cassette, the liquid will laterally flow through the surface of the test strip and react with the pre-coated Brucella antibody. If there are anti-Brucella antigens in the specimen, a visible T line will appears. The C line should always appear after a sample is applied, which indicates a valid result. By this means, the cassette can accurately indicate the presence of Bovine Brucella antigens in the specimen.

How to use:

• Collect fresh whole blood, or separate serum or plasma from blood as soon as possible to avoid hemolysis. Only use clear, non-hemolyzed specimens. Do not leave the specimens at room temperature for prolonged periods. Serum and plasma specimens may be stored at 2-8 °C for up to 3 days. For long term storage, serum and plasma specimens should be kept below -20 °C.
• Place the test cassette on a clean and level surface.

For serum and Plasma: Hold the dropper vertically, draw the specimen about 1cm above the Fill Line, and transfer 2 drops of serum or plasma (approximately 40μl) to the specimen well (S), then add 2 drops of buffer (approximately 120 μl) into the buffer well (B). See illustration below and start the timer.

For whole blood specimen: Hold the dropper vertically, draw the specimen about 1cm above the Fill Line, and transfer 1 drop of whole blood (approximately 20μl) to the specimen well (S), then add 2 drops of buffer (approximately 120 μl) into the buffer well (B). See illustration below and start the timer.

• Read the result at 10 minutes. Do not interpret results after 20 minutes.
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