Principle of the procedure

This kit is based on colloidal gold immunochromatography and uses double-antigen sandwich assay to detect novel neutralizing antibodies against coronavirus in peripheral whole blood. The T line of the novel coronavirus neutralizing antibody test cassette was coated with recombinant novel coronavirus S-RBD antigen, and the C line was coated with sheep anti-S-RBD polyclonal antibody. During detection, the samples were dripped into the sample well, and the neutralizing antibodies of the novel coronavirus was combined with the recombinant S-RBD antigen of the novel coronavirus labeled with colloidal gold, a solid phase the recombinant S-RBD antigen of the novel coronavirus - novel coronavirus neutralizing antibodies - labelled the recombinant S-RBD antigen of the novel coronavirus - colloidal gold complex at the T line position, solid-phase sheep anti-S-RBD polyclonal antibody - the recombinant S-RBD antigen of the novel coronavirus-colloidal gold complex was formed at the C line.

Test procedure

Please read the instruction for use carefully before testing, and complete the test in strict accordance with the directions of the manual, otherwise reliable results cannot be guaranteed.

1. Place the test cassette, sample diluent and test sample for 15~30 minutes, and equilibrate to room temperature (10~30℃).

2. Open the aluminum foil pouch of the test cassette, and place the test cassette on a flat surface.

3. Write sample ID on the plastic case of the test cassette.

4. Peripheral whole blood samples:

1). Tear off the disposable alcohol pad, wipe your finger, and dry naturally.

2). Using the lancet, pull out the lid of the lancet, press it on the finger that has been disinfected, and puncture the finger with the lancet.

3). Squeeze out the first drop of whole blood and wipe it off with a cotton swab.

4). Squeeze out the second drop of whole blood, and use a dropper to draw the whole blood above the mark.