Nitrofuran AHD Diagnostic ELISA Kit for Aquatic Products 96Wells/Kit

1. Nitrofuran AHD Diagnostic ELISA Kit Principle

This kit is based on a competitive enzyme immunoassay for the detection of aminohydantoin (AHD) in samples. Conjugated antigens are pre-coated on microwell strips. Aminooxalic acid (AHD) in the sample competes with the conjugated antigen pre-coated on the microwell strip for anti-AHD antibodies. After the enzyme conjugate is added, TMB substrate is added for staining. The optical density (OD) value of a sample is negatively correlated with the AHD in it. This value is compared to a standard curve and the AHD concentration is subsequently obtained.

2. Technical Specifications

Sensitivity: 0.04ppb
Incubation temperature: 25℃
Incubation time: 30min～15min
Detection limit tissue, eggs, honey: 0.1ppb
cross-reaction rate
AHD 100%
AMOZ < 0.1%
AOZ < 0.1%
SEM < 0.1%
recovery rate
Tissue, eggs 95±25%
Honey 75±25%

3. Nitrofuran AHD Diagnostic ELISA Kit Components

4. Sample preparation

Tissue​, eggs
1) Weigh 1±0.05g of homogeneous sample, add 4mL of distilled water, 0.5mL of 1 M HCl and 100µL of 2-nitrobenzaldehyde (C7H5NO3) to each tube, and shake appropriately for 2min;
2) Incubate overnight at 37°C (about 16 hours) or in a water bath at 56°C (2 hours).
3) Add 5mL 0.1M K2HPO4, 0.4mL 1M NaOH and 6mL ethyl acetate to each tube, shake well for 30s.
4) Centrifuge at room temperature (20-25°C) above 4000r/min for 10min (if there is emulsification or the ethyl acetate layer is less than 3ml, incubate the sample in a water bath at 80°C for 10min, and centrifuge repeatedly; or increase the speed and prolong the centrifugation time).
5) Transfer 3 mL of ethyl acetate layer to a new centrifuge tube and evaporate to dryness with nitrogen or air at 50°C.
6) Dissolve the dry residue with 2 mL of n-hexane, add 1 mL of the diluted reconstituted solution, mix well for 30 seconds, and centrifuge at room temperature (20-25°C) at 4000 rpm for 10 minutes; remove the upper n-hexane phase (if emulsified) phenomenon, after removing the upper n-hexane phase, the samples were incubated in a 70°C water bath for 10-20min, and centrifuged repeatedly).
7) Take 50 µL of the lower layer for analysis.
Sample dilution factor: 2

5. matters needing attention

1) If the room temperature is lower than 25°C or the reagent temperature and the sample do not return to room temperature (20-25°C), the standard OD value will be low.
2) During the washing process, the drying of the microplate will be accompanied by the nonlinearity of the standard curve, and the reproducibility is not ideal; therefore, proceed to the next step immediately after washing.
3) Mix well, otherwise the reproducibility will be poor.
4) The stop solution is 2M sulfuric acid solution, avoid contact with skin.
5) Do not use the kit beyond the shelf life. The use of diluted or adulterated reagents from the kit can result in changes in sensitivity and detection OD values. Do not replace reagents in different batches of kits.
6) Reseal unused microplates in ziplock bags. Standard solutions and colorless couplers are light-sensitive and cannot be directly exposed to light.
7) Discard any tinting solution whose color indicates that the solution has been denatured. A detection value of less than 0.5 for Standard Solution 1 (0 ppb) indicates degradation.
8) The optimal reaction temperature is 25°C, and the detection sensitivity and OD value will change if the temperature is too high or too low.

6. Storage and validity period

Storage: Store at 2 to 8°C, do not freeze.
Expiration date: 12 months; production date is on the box.
Note: If the microplate vacuum packaging leaks, it can still be used without affecting the test results, please use it with confidence.

Q1: When will it be shipped?
A1: We will ship the goods for you as soon as possible within 7 working days after receiving the payment. (In case of external factors such as the epidemic, the delivery may be delayed)

Q2: Does it support OEM/ODM?
A2: It can be supported, but the specific quantity needs to be more than 100,000 pieces, which is convenient for customized products.

Q3: How is your factory doing in terms of quality control?
A3: We have nationally certified ISO9001 and ISO13485. Our production process conforms to standard procedures to ensure optimum product quality.

Q4: How to provide after-sales service?
A4: We provide professional online technical after-sales service. We can provide you with one-on-one guidance via video, telephone, etc.

Q5: What is the payment method?
A5: We receive payment by T/T.

Q6: How to ship?
A6: Choose the best shipping method for you by getting quotes from our many cooperative carriers, and also ship according to your requirements.