Nitrofuran AMOZ Rapid Test Strip for Tissue 96 Tests/Kit 0.5 ppb

1. Specifications

96 strips/kit

2. Brief

This product is used for testing Nitrofuran(AMOZ) in Chicken, Aquatic Products (Fish, Shrimp, Crab) sample qualitatively. Easy to operate, high sensitivity.

3. Detection limit

Tissue: 0.5ng/g (0.5ppb)

4. Contents

5. Principle

Nitrofuran (AMOZ) rapid test strips are based on the principle of competitive inhibition immunochromatography. During the flow process, nitrofuran (AMOZ) in the sample binds to nitrofuran (AMOZ)-specific colloidal gold-labeled monoclonal antibody, which inhibits the conjugation of the antibody to nitrofuran (AMOZ)-BSA on the NC membrane detection line combination of things. combination, resulting in a change in the color test line. When the sample has no nitrofuran (AMOZ) residue or the concentration is lower than the detection limit, the T line is darker than the C line or the T line is the same color as the C line; when the concentration is equal to or higher than the detection limit, the T line is obviously lighter than the C line or the T line Lines have no color. The C line appears regardless of the presence or absence of nitrofuran (AMOZ) residues in the sample, indicating that the test is valid.

6. Sample preparation

6.1 Solution Preparation
0.1MK2HPO4 solution: Dilute 1M K2HPO4 solution 1:9 with deionized water.

6.2 Sample Handling
Tissue:
1) Take 3±0.05g of homogenized tissue sample into a 50ml centrifuge tube, add 6ml of deionized water, 750ul of 1M HCl and 300ul of derivatization reagent, shake well for 2min, and take a 60°C water bath for 30min;
2) Add 7.5ml 0.1M K2HPO4 solution, 600ul 1M NaOH and 10ml ethyl acetate, shake well for 1min, and centrifuge at 4000r/min for 10min;
3) Take 5ml of the supernatant in a glass tube and dry it at 56°C with nitrogen or air.
4) Add 2ml of n-hexane, shake well to dissolve the dry residue (if the residue is not completely dissolved, the result may be unstable), then add 0.6ml of sample buffer, shake well for 30s, stabilize until layered, then absorb the upper layer (if it sees milky white). liquid, centrifuge at 4000r/min for 5min);
5) Aspirate 200ul of the lower liquid (water layer) and prepare for testing. (Avoid absorbing upper liquid).

7. Operation procedures

7.1 Read the instruction carefully before use. Return test dipsticks and sample into room temperature.
7.2 Take bottles needed from the kit package, take out required microwells and dipsticks, making proper marks. Please use these test dipsticks within 1h. Seal the cap of the bottles, avoid moisture.
7.3 Take 200ul of the test samples into the microwells, then repeatedly absorb for 5-10 times, mix the sample with the reagent in the microwells completely until no solid judged by eyes (this is a very important step).
7.4 Incubate for 5min at room temperature (20-25℃), insert the test dipsticks into the microwells with the "MAX" end fully dipped in to the mixture solution.
7.5 Incubate for 5-8min at room temperature again, read the result, it is invalid in other time.

8. Test Result Interpretation

8.1 Negative: T line is darker than C line, or T line has same color with C line. It means there is no Nitrofuran(AMOZ) residue in sample or the residue is lower than detection limit.
8.2 Positive: T line is obviously lighter than C line or T line is invisible. It means the Nitrofuran(AMOZ) residue is equal to or higher than detection limit.
8.3 Invalidation: C line isn’t seen wine red. It means the test card is out of efficacy, out of date or improper operation. Please run the test again using another package. If the invalid tests keep happening, please contact the supplier.

9. Precautions

1) The test card can only be used once at room temperature, please do not use the expired test card.
2) Do not reuse tips to avoid cross contamination.
3) Do not touch the white film surface in the middle of the test card, avoid direct sunlight and direct blowing of fans.
4) Wear gloves when testing.
5) If you have any questions, please contact the supplier.

10. Specificity

No cross-reactivity with nitrofurans AOZ, AHD and SEM.

11. Storage and expiration date

Storage: 2-8 ℃ dark, sealed, dry place, not frozen.
Expiration date: 12 months; production date is on the box.

Q1: When will it be shipped?
A1: We will ship the goods for you as soon as possible within 7 working days after receiving the payment. (In case of external factors such as the epidemic, the delivery may be delayed)

Q2: Does it support OEM/ODM?
A2: It can be supported, but the specific quantity needs to be more than 100,000 pieces, which is convenient for customized products.

Q3: How is your factory doing in terms of quality control?
A3: We have nationally certified ISO9001 and ISO13485. Our production process conforms to standard procedures to ensure optimum product quality.

Q4: How to provide after-sales service?
A4: We provide professional online technical after-sales service. We can provide you with one-on-one guidance via video, telephone, etc.

Q5: What is the payment method?
A5: We receive payment by T/T.

Q6: How to ship?
A6: Choose the best shipping method for you by getting quotes from our many cooperative carriers, and also ship according to your requirements.