Nitrofuran SEM Rapid Test Strip for Tissue 96 Tests/Kit 0.5 ppb

1. Specifications

96 strips/kit

2. Brief

This product is used for testing Nitrofuran(SEM) in Chicken, Aquatic Products (Fish, Shrimp, Crab) sample qualitatively. Easy to operate, high sensitivity.

3. Detection limit

Tissue: 0.5ng/g (0.5ppb)

4. Contents

5. Principle

Nitrofuran (SEM) rapid test strips are based on the principle of competitive inhibition immunochromatography. During the flow process, the nitrofuran (SEM) in the sample binds to the nitrofuran (SEM)-specific colloidal gold-labeled monoclonal antibody, which inhibits the antibody and the nitrofuran (SEM)-BSA conjugate at the NC membrane detection line On the combination, resulting in a change in the color test line. When the sample has no nitrofuran (SEM) residue or the concentration is lower than the detection limit, the T line is darker than the C line or the T line is the same color as the C line; when the concentration is equal to or higher than the detection limit, the T line is obviously lighter than the C line or the T line Lines have no color. The C line appears regardless of the presence or absence of nitrofuran (SEM) residues in the sample, indicating that the test is valid.

6. Sample preparation

6.1 Solution preparation
0.1MK2HPO4 solution: Dilute1M K2HPO4 solution with deionized water at 1:9.

6.2 Sample treatment
Tissue:

1) Take 3±0.05g of homogenized tissue sample into a 50ml centrifuge tube, add 6ml of deionized water, 750ul of 1M HCl and 300ul of derivatization reagent, shake well for 2min, and take a 60°C water bath for 30min;
2) Add 7.5ml 0.1M K2HPO4 solution, 600ul 1M NaOH and 10ml ethyl acetate, shake well for 1min, and centrifuge at 4000r/min for 10min;
3) Take 5ml of the supernatant liquid into a glass tube, and dry it with nitrogen or air at 56℃.
4) Add 2ml of n-hexane and shake well to dissolve the dry residue (if the residue is not completely dissolved, the result may be unstable), then add 0.6ml of sample buffer, shake well for 30s, stabilize until stratified, and absorb the upper layer (if it appears milky). liquid, centrifuged at 4000r/min for 5min);
5) Absorb 200ul of the lower liquid (water layer) and prepare for testing. (Avoid absorbing upper liquid).

7. Operation procedures

7.1 Please read the manual carefully before use. Return the test dipstick and sample to room temperature.
7.2 Take the required bottle from the kit, take out the required micropore and dipstick, and make a mark. Please use these test strips within 1 hour. Seal the cap to avoid moisture.
7.3 Take 200ul of the sample to be tested in the microwell, absorb it 5-10 times, and mix the sample with the reagent in the microwell until it is judged by the naked eye that there is no solid (this is a very important step).
7.4 Incubate at room temperature (20-25°C) for 5 minutes, insert the test paper into the microwell, and completely immerse the "MAX" end in the mixed solution.
7.5 Incubate again at room temperature for 5-8min, read the result, other time is invalid.

8. Test Result Interpretation

8.1 Negative: T line is darker than C line, or T line has same color with C line. It means there is no Nitrofuran(SEM) residue in sample or the residue is lower than detection limit.
8.2 Positive: T line is obviously lighter than C line or T line is invisible. It means the Nitrofuran(SEM) residue is equal to or higher than detection limit.
8.3 Invalidation: C line isn’t seen wine red. It means the test card is out of efficacy, out of date or improper operation. Please run the test again using another package. If the invalid tests keep happening, please contact the supplier.

9. Precautions

1) The test card can be used only once at room temperature, do not use test card out of expiry date.
2) Do not repeat use the tips to avoid cross pollution.
3) Do not touch the white membrane surface in the middle of test card, avoid sunlight and fan blowing directly.
4) Wear gloves when testing.
5) Please contact the supplier for any questions.

10. Specificity

There is no cross reaction with Nitrofuran AMOZ, AOZ and AHD.

11. Storage and expiry date

Storage: Store at 2-8 ℃ in dark, sealed, dry place, no frozen.
Expiry date: 12 months; date of production is on box.

Q1: When will it be shipped?
A1: We will ship the goods for you as soon as possible within 7 working days after receiving the payment. (In case of external factors such as the epidemic, the delivery may be delayed)

Q2: Does it support OEM/ODM?
A2: It can be supported, but the specific quantity needs to be more than 100,000 pieces, which is convenient for customized products.

Q3: How is your factory doing in terms of quality control?
A3: We have nationally certified ISO9001 and ISO13485. Our production process conforms to standard procedures to ensure optimum product quality.

Q4: How to provide after-sales service?
A4: We provide professional online technical after-sales service. We can provide you with one-on-one guidance via video, telephone, etc.

Q5: What is the payment method?
A5: We receive payment by T/T.

Q6: How to ship?
A6: Choose the best shipping method for you by getting quotes from our many cooperative carriers, and also ship according to your requirements.