Fumonisin B1 ELISA Test Kit for Feed Peanut Maize 96 Wells/Kit

1. Principle

This test kit is based on the indirect competitive enzyme immunoassay for the detection of Fumonisin B1. The coupling antigen is pre-coated on the micro-well stripes. The Fumonisin B1 in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti- Fumonisin B1 antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Fumonisin B1 in the sample. This value is compared to the standard curve and the Fumonisin B1 residues is subsequently obtained.

2. Technical specifications

Sensitivity: 0.5ppb
Incubator temperature: 25℃
Incubator time: 30min～15min
Detection limit:
Feed, peanut, rice, maize 25ppb
Cross-reaction rate:
Fumonisin B1 100%
Recovery rate:
Feed, peanut, rice, maize 95±35%

3. Components

4. Sample pre-treatment

Instructions(The following points must be dealt with before the pre-treatment)
1) Only the disposable tips can be used for the experiments and the tips must be changed when used for absorbing different reagents;
2) Before the experiment, each experimental utensil must be clean and should be re-cleaned if necessary, in order to avoid the contamination that interferes with the experimental results.

Solution preparation before sample pre-treatment:
1) Sampleredissolving solution
Use 1 part of 2Xconcentrated redissolving solution and dissolve with 1 part of deionized water to obtain the ready to use sample redissolving solution.
2) Sample extract solution
Use 7 parts of Methanol and dissolve with 3 parts of deionized water to obtain the ready to use sample extract solution.

Samples Preparation
4.1 Preparation of Feed, maize sample
1) Take 1.0±0.05g grinded feed or maize sample into 50ml centrifuge tube, add 5ml sample extract solution, shake for 3min, centrifuge at above 4000r/min at 20℃ for 10min;
2) Take up-layer clear liquid 100ul, add 900ul deionized water, shake to evenly;
3) Take 50μl to test
Dilution factor:50
Note: if measured sample content is beyond the curve range, dilute the sample for many times (for example: take 50ul up-layer clear liquid into a new centrifuge tube, add 950ul deionized water, then the dilution factor is 100)

4.2 Preparation of Rice sample
1) Take 1.0±0.05g grinded rice sample into 50ml centrifuge tube; add 5ml sample extract solution, shake for 3min, centrifuge at above 4000r/min at 20℃ for 10 min;
2) Take 100ul up-layer clear liquid, add 900ul sample redissolving solution, shake to evenly;
3) Take 50μl to test
Dilution factor:50
Note: if measured sample content is beyond the curve range, dilute the sample for many times (for example: take 50ul up-layer clear liquid into a new centrifuge tube, add 950ulsample redissolving solution, then the dilution factor is 100)

4.3 Preparation of Peanut sample
1) Take 1.0±0.05g grinded peanut sample into 50ml centrifuge tube; add 5ml sample extract solution, then add 4ml N-hexane, shake for 3min, centrifuge at above 4000r/min at 20℃ for 10 min;
2) Discard up-layer clear liquid, take middle-layer liquid 100ul, add 900ul deionized water, shake to evenly;
3) Take 50μl to test
Dilution factor:50
Note: if measured sample content is beyond the curve range, dilute the sample for many times (for example: take 50ul middle-layer liquid into a new centrifuge tube, add 950ul deionized water, then the dilution factor is 100)

5. ELISA procedures

5.1 Instructions
1) Bring ELISA reagents to room temperature (20 - 25 °C) before use.
2) Put ELISA reagents back to 2-8 ℃immediately after use
3) The ELISA reproducibility in the analysis process is largely depends on the consistency of the washing plate, the correct washing plate operation is the point of determination ELISA program
4) In all process of constant temperature incubation, avoid light exposure, seal the microplate with the cover membrane

5.2 Operation procedures
1) Bring test kit to the room temperature (20-25 ℃) for at least 30 min, note that each reagent must be shaken evenly before use;
2) Put the required micro-well strips into plate frames. Re-sealed the unused microplate, stored at 2-8 ℃, not frozen.
3) Solution preparation: take the 40ml 20× concentrated washing buffer, dissolve with deionized water at 1:19 (1 part 20× concentrated washing buffer + 19 parts deionized water), or prepare as quantity needed.
4) Numbering: number the micro-wells according to samples and standard solution; each sample and standard solution should be performed in duplicate; record their positions.
5) Add standard/sample: Add 50 µL of the sample or the standard solution into separate duplicate wells, then add enzyme conjugate, 50 µL/well; then antibody working solution, 50 µL/well. Mix gently by shaking the plate manually, seal the microplate with the cover membrane,incubate at25 °C for 30 min in the dark.
6) Wash microplate: Carefully open the cover membrance, pour liquid out of microwell; add 250 µL/well of washing buffer, wash fully for 4-5 times, 15-30 s each time, then take out and flap to dry with absorbent paper.(Use unused spear to pierce bubble after dry)
7) Coloration: add 50 µL of substrate A solution then 50 µL B solution into each well. Mix gently by shaking the plate manually, andincubate at25 °C for 15minin the dark for coloration.
8) Determination: add 50 µL of the stop solution into each well. Mix gently by shaking the plate manually. Set the wavelength of the microplate reader at 450 nm to determine the OD value of every well. (Recommend to read the OD value at the dual-wavelength 450/630 nm within 5 min).

Q1: When will it be shipped?
A1: We will ship the goods for you as soon as possible within 7 working days after receiving the payment. (In case of external factors such as the epidemic, the delivery may be delayed)

Q2: Does it support OEM/ODM?
A2: It can be supported, but the specific quantity needs to be more than 100,000 pieces, which is convenient for customized products.

Q3: How is your factory doing in terms of quality control?
A3: We have nationally certified ISO9001 and ISO13485. Our production process conforms to standard procedures to ensure optimum product quality.

Q4: How to provide after-sales service?
A4: We provide professional online technical after-sales service. We can provide you with one-on-one guidance via video, telephone, etc.

Q5: What is the payment method?
A5: We receive payment by T/T.

Q6: How to ship?
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