Tuberculosis Antibody Diagnostic ELISA Kit for Cattle Sheep 96 Wells/Kit

1. Tuberculosis Antibody Diagnostic ELISA Kit Introduction

Mycobacterium tuberculosis (Mycobacterium bovis) antibody ELISA kit is used to detect Mycobacterium tuberculosis antibodies in bovine serum. Serology-assisted diagnosis for the evaluation of tuberculosis caused by Mycobacterium bovis.
This kit adopts the indirect ELISA method to coat the Mycobacterium tuberculosis antigen on the enzyme microporous strip. During the detection, add the diluted serum sample. After incubation, if there are Mycobacterium tuberculosis-specific antibodies bound to the coating antigen, the unbound antibodies and other components are washed and discarded; The complexes with pre-coated antigens are bound; the unbound enzyme conjugates are discarded by washing; TMB substrate is added to the microwells to enzymatically form blue products, after adding stop solution to stop the reaction, use a microplate reader at 450nm The absorbance A value of the reaction well was measured at the wavelength.

2. Reagents and contents

3. Test procedure

1) Take the tuberculosis-silver-coated plate (open and measure the required amount according to the sample), add the diluted serum to the test wells, 100ul/well; at the same time, set 2 wells of positive control and 1 well of negative control, respectively in their wells Add negative control and positive control, 100ul/well (no overflow);
2) Cover with self-adhesive foil and incubate at 37°C for 30 minutes;
3) Open the film, discard the well solution, add diluted washing buffer to each well, 250ul/well, discard the solution, repeat the above steps 4-6 times, and finally pat dry with absorbent paper;
4) Add Enzyme Conjugate, 100ul/well, shake gently, cover with plastic wrap, and incubate at 37°C for 30 minutes;
5) Open the self-adhesive foil, pour out the liquid in the well, add diluted washing buffer to each well, 250ul/well, pour out the liquid, repeat the above steps 4-6 times, and finally pat dry with absorbent paper;
6) Add substrate, 100ul/well, mix well, cover with plastic wrap, and incubate at 37°C for 10 minutes in the dark;
7) Add stop solution, 50ul/well to stop the reaction, and measure the result in 10 minutes.

4. Result judgment

Read the OD value at 450nm (630nm as reference).
For the detection to work:
Negative control OD value (N)<0.2, while positive control OD value (P)>0.4
Calculation method:
S/P value = sample OD value/positive control OD average
Interpretation of results
S/P value < 0.3: negative
S/P value) ≥ 0.3: positive

5. Storage and Expiration Dates

Store in the dark at 2~8°C, do not freeze, shelf life: 12 months.

Q1: When will it be shipped?
A1: We will ship the goods for you as soon as possible within 7 working days after receiving the payment. (In case of external factors such as the epidemic, the delivery may be delayed)

Q2: Does it support OEM/ODM?
A2: It can be supported, but the specific quantity needs to be more than 100,000 pieces, which is convenient for customized products.

Q3: How is your factory doing in terms of quality control?
A3: We have nationally certified ISO9001 and ISO13485. Our production process conforms to standard procedures to ensure optimum product quality.

Q4: How to provide after-sales service?
A4: We provide professional online technical after-sales service. We can provide you with one-on-one guidance via video, telephone, etc.

Q5: What is the payment method?
A5: We receive payment by T/T.

Q6: How to ship?
A6: Choose the best shipping method for you by getting quotes from our many cooperative carriers, and also ship according to your requirements.