CPV Canine Parvovirus Antigen Rapid Test Kit for Pet 10Tests/Kit GMP ISO9001
Basic overview of CPV
In 1977, Eugster first found canine parvovirus in the feces of young dogs suffering from diarrhea through electron microscopy. By 1979, canine parvovirus enteritis or myocarditis had almost spread all over the world. Since 1980, there have been reports of this disease in China. Canine parvovirus is highly contagious to dogs and can be infected by dogs of all ages. However, dogs from weaning to 90 days old have more and more serious diseases. Some puppies may present symptoms of myocarditis and die suddenly. According to the types of clinical dogs, purebred dogs and exotic dogs have a higher incidence rate than native dogs. This disease can occur all the year round, but it occurs frequently in cold seasons. The feces of sick dogs contain the highest amount of poison. Dogs infected with parvovirus can be divided into enteritis type and myocarditis type in clinic. The initial symptoms were fever (above 40 ℃), depression, anorexia, vomiting and diarrhea. At the beginning of the disease, the stool was thin, and with the development of the disease, the stool was brown or tomato sauce-like bloody stool with a special fishy smell. Severe dehydration in the later stage. Myocarditis type is mostly seen in dogs around 40 days old. Some dogs can die after mild diarrhea.
1. Product Usage
Canine parvovirus (CPV) disease is a potent canine infection that is highly contagious. The pathogen is canine parvovirus, mainly CPV-2. The virus mainly infects dogs, especially puppies, which are highly contagious, have an acute onset, and have a high mortality rate. The disease is usually caused by intestinal infection, with fecal-oral infection as the main route of transmission, and susceptible animals are mainly infected by direct or indirect contact. This product is tested by immunochromatography on canine feces or vomit samples to quickly detect whether a dog has been infected with canine parvovirus.
2. Principle
This product uses rapid immunochromatography to detect CPV antigen. After the diluted sample is added to the“S” hole on test card, if the CPV antigen is present in the sample, the antigen will specifically bind to the colloidal gold-labeled CPV monoclonal antibody, and the complex will move along the chromatographic membrane and be chromatographed. The pre-coated CPV monoclonal antibody on the membrane is captured, and a wine red detection line is formed at the T-position of the cartridge. If there is no CPV antigen in the sample, no visible lines will be formed at the T line. In addition, the system has also designed a C-line for verifying that the test is valid—whether negative or positive, the line should be colored, otherwise it will be invalid.
3. CPV Pet Rapid Test Components
CPV Ag Test Card | 10 pieces |
Sample buffer | 10 tubes |
Swab | 10 pieces |
Instruction | 1 piece |
4. Test Procedure
1)Read the manual carefully and return the test card, sample into room temperature 15~25℃before use.
2)Sample collection: Use a cotton swab to collect a sample of freshly excreted feces or vomit, or take a sample directly from the rectum. Immediately insert the cotton swab with the secretion into the sample tube containing the sample buffer and shake vigorously. Stand for a few minutes and ready to test. (Note: When the sampling amount is too much, it will affect the stability of the colloidal gold particles, which may cause false positive. The sample size should cover 1/3 to 2/3 of the cotton swab.)
3)Open the package, take out test card, put it on desk flatly.
4)Use the supplied dropper to absorb the diluted sample, drop 3~4 drops(about 100ul)into“S”hole on test card.
5)Read the result in 5~10min, result is invalid beyond 15 min.
5. Result judgment
1) Positive(+): Both on T Line and C line being seen wine-red color reaction, it means there is CPV antigen in the sample.
2) Negative(-): No color reaction on Test Line (T Line),only on Control Line(C Line) being seen wine-red color reaction, it means there is no CPV antigen in the sample.
3) Invalid(X): No color reaction on C Line, it means the operation is wrong or the test card is invalid, test again.
6. Specifications, Storage and Stability
Package: 10T/box
Store at 4~30℃ in a cool, dark and dry place,no frozen.
Shelf life: 18 months.
7. Precautions
1) Please use the test card within the shelf life and within 1 hour after opening.
2) Avoid direct sunlight and direct fan blowing during testing.
3) Try not to touch the white film surface of the non-plastic part of the test card.
4) Sample droppers should not be mixed to avoid cross contamination.
5) Do not use sample buffer that are not supplied with this reagent.
6) When the detected substance content is very high, the T line may appear, and then the red color will gradually fade or even disappear. It is recommended to dilute the sample by 10 times before testing, so that the T line can be stable.
7) The used test card should be treated as microbiological dangerous goods.
8. Application limitations
This product is an immunological diagnostic kit, which is only used to provide qualitative test results for clinical testing of pet diseases. If there is any doubt about the test results, please use other methods of diagnosis (such as RT-PCR, Western-blot, etc.) to further analyze the samples. For pathological analysis, please consult your local pet physician.
Q1: How long will it take for you to ship?
A1: Usually ships within 7 working days.
Q2: Do you support OEM/ODM?
A2: can be supported. We can customize according to your specific needs and specific quantities.
Q3: How is your factory doing in terms of quality control?
A3: We have ISO9001 certification and ISO13485 certification, so far all the production process, we have standard rules, we comply with the relevant behavior and laws of the government.
Q4: Is after-sales service guaranteed?
A4: We provide professional online technical after-sales service. If the product fails during the experiment, we can provide
one-to-one guidance through telephone, video and other forms.
Q5: What is your minimum order quantity?
A5: 1Kit.
Q6: What is the shipping method?
A6: It can be shipped by express (FEDEX, UPS, DHL, EMS, etc.) or by air and ground.Please confirm with us before placing an order.