Avian Influenza H5N1 Antibody ELISA Test Kit for Poultry 96 Wells/Kit
1. Usage
This kit is used to detect Avian Influenza Virus H5N1 antibody in chicken serum, to assess antibody condition by Avian Influenza disease vaccine in chicken farm and assist diagnosis of serological infected chicken.
2. Principle
The Avian Influenza Virus H5N1 antibodyELISA kit is based on an indirect enzymatic immunoassay (Indirect ELISA).The antigen is coated on plates. When a sample serum contains specific antibodies against virus, they will bind to the antigen on plates. Wash the unbound antibodies and other components. Then add a specific enzyme conjugate. After incubation and washing, add the TMB substrate. A colorimetric reaction will appear, measured by a spectrophotometer (450 nm).
3. Reagents
H5N1 antigen coated microtiter strips | 1 piece (96 wells) |
Conjugate solution | 1 bottle (12 mL/bottle) |
10× washing solution | 1 bottle (50 mL/bottle) |
Substrate A /B solution | 1 tube each (6 mL/tube) |
Sample dilution | 1 bottle (50 mL/bottle) |
Stopping solution | 1 tube (6 mL/tube) |
Negative control | 1 tube (800μL /tube) |
Positive control | 1 tube (800μL /tube) |
Serum diluent plate | 1 piece |
Adhesive film | 2 pieces |
Instruction | 1 piece |
4. Materials required but not provided
1) Micropipettors and disposable tips: 0.5μL~10μL,10μL~100μL,100μL~1000μL
2) 37 °C Incubator
3) Measuring cylinder: 500 ml
4) 96 wells microplate reader
5) Distilled water or deionied water
6) Bottle or microplate washing machine
5. Sample preparation
Take animal whole blood, make serum according to regular methods, the serum should be clear, without hemolysis.
Q1: How long will it take for you to ship?
A1: Usually ships within 7 working days.
Q2: Do you support OEM/ODM?
A2: can be supported. We can customize according to your specific needs and specific quantities.
Q3: How is your factory doing in terms of quality control?
A3: We have ISO9001 certification and ISO13485 certification, so far all the production process, we have standard rules, we comply with the relevant behavior and laws of the government.
Q4: Is after-sales service guaranteed?
A4: We provide professional online technical after-sales service. If the product fails during the experiment, we can provide
one-to-one guidance through telephone, video and other forms.
Q5: What is your minimum order quantity?
A5: 1Kit.
Q6: What is the shipping method?
A6: It can be shipped by express (FEDEX, UPS, DHL, EMS, etc.) or by air and ground.Please confirm with us before placing an order.