Swine Foot and Mouth Disease Virus Antibody Rapid Test Kit 50 Tests/Kit

1. Principle

This rapid test is an immuno-chromatographic test, using colloidal gold immunoassay method, to detect the antibody against FMD virus in whole blood, serum. After adding sample, the sample move along with the colloidal gold labeling FMD antigen, is there is FMD antibody in the sample, it combines with antigen on T line and show wine-red color; if there is no FMD antibody, then no color reaction.

2. Components

3. Test procedure

1) Collect whole blood, separate serum (centrifuge at 2000-3000 r/min for 5~15min or separate out naturally at 4℃overnight), or take whole blood with anticoagulants as sample. Take whole blood without anticoagulants as sample is also OK, but must test it immediately.
2) Open the package, take out test card, put it on clean, flat desk.
3) Use dropper to absorb the sample, adding 3 drops sample into “S” mark well slowly drop by drop.
4) Judge the result in 10-20min, it is invalid beyond 20min.

4. Results judgment

1) Positive: control line and test line are both seen wine red, the more the antibody exist, the thicker the color appear;

2) Weak positive: control line and test line are both seen wine red, but the color of test line is very light;

3) Negative:only control line is seen wine red;

4) Invalidation: control line isn’t seen wine red.

5. Consults for diagnosis

1) If not inoculated by the bacterin against porcineFMD virus:
a. test line don’t appear visible wine red show there are no antibodies against porcine FMD virus in tested samples, if the swine are healthy, it is better to vaccinate in time;
b. test line appear visible wine red show the pig was infected with wild virus, should be more observed to validate.

2) if have been inoculated by the bacterin against porcineFMD virus:
a. the color of test line is thicker than the color of the titer 1:32 from comparison card show the high titer of the antibody against porcine FMD virus, which is enough to resist the strong virus;
b. the color of test line is thinner than the color of the titer 1:32 from comparison card show the the titer of the antibody against porcine FMD virus is not enough to resist the strong virus, it is better to perform complementary vaccination.

6. Storage and expiry date

Store at 2 - 30ºC and the shelf life is 24 months.

7. Notice

1) Do not use expired or poorly packaged test cards;
2) Do not use purified water, tap water and normal saline as negative controls;
3) Store at room temperature (< 30°C). If it is stored in a cool place, it is necessary to return to normal temperature first, and then open the package to avoid moisture. Use the test card as soon as possible after opening the package.
4) Do not touch the white film surface in the middle of the test card.
5) To ensure the quality of the test, please take fresh, non-contaminated samples. If there is a lot of blood lipids, it will affect the results, so after centrifugation, take the serum test to avoid fat. If the blood or serum is viscous or not viscous enough, it can be diluted with normal saline at the same dilution and then tested.
6) If the serum cannot be detected immediately, it can be stored at 4°C for a short time (within 48 hours) and for a long time at -20°C.
7) Whole blood without anticoagulant should be tested immediately; if some Krull is already present, separate serum for testing. Whole blood containing anticoagulants should be tested within 24 hours and not frozen. Freezing and thawing lead to hemolysis and rupture of red blood cells, which seriously affects the test results.
8) When adding the sample dropwise, keep the pipette tip and the "S" hole 1cm to ensure accurate sample addition. If it is too close, it will cause the droplet volume to be too small, resulting in wrong judgment of the droplet or not allowing the amount of sample to be added, which will affect the test results.
9) Avoid foaming when adding samples, and the sample volume should be controlled at about 100ul (about 3 drops). Too many samples will not increase sensitivity. In particular, excess whole blood causes erythrocytes to settle in the "well", causing membrane chromatography to penetrate deep into the red background, which seriously affects the results.
10) This test card is only for pig samples, and whether it is suitable for other animals has not yet been evaluated.
11) Antibody level is only related to T-line darkness, not C-line. Only the C wire is clean, indicating that the test is valid.
12) Dispose of the test cards and samples at the contaminated site according to local regulations.

Q1: How long will it take for you to ship?
A1: Usually ships within 7 working days.

Q2: Do you support OEM/ODM?
A2: can be supported. We can customize according to your specific needs and specific quantities.

Q3: How is your factory doing in terms of quality control?
A3: We have ISO9001 certification and ISO13485 certification, so far all the production process, we have standard rules, we comply with the relevant behavior and laws of the government.

Q4: Is after-sales service guaranteed?
A4: We provide professional online technical after-sales service. If the product fails during the experiment, we can provide
one-to-one guidance through telephone, video and other forms.

Q5: What is your minimum order quantity?
A5: 1Kit.

Q6: What is the shipping method?
A6: Usually by air, we will choose the best shipping method for you, and we can also ship according to the shipping method you require.