Nitrofuran SEM Diagnostic ELISA Kit for Shrimp 96 Wells/Kit Sensitivity 0.02

1. Nitrofuran SEM Diagnostic ELISA Kit Principle

This test kit is based on a competitive enzyme immunoassay for the detection of nitrofurans (SEM) in samples. Conjugated antigens are pre-coated on microwell strips. The nitrofuran (SEM) in the sample competes with the bound antigen pre-coated on the microwell strip for anti-SEM antibodies. After adding the enzyme conjugate, add TMB substrate for staining. The optical density (OD) value of a sample is negatively correlated with the SEM in it. This value was compared to a standard curve and the SEM concentrations were subsequently obtained.

2. Technical specifications

Sensitivity: 0.02ppb
Incubation Temperature: 25℃
Incubation Time: 30min～15min
Detection limit Tissue, egg, honey: 0.1ppb

Detection limit Feed:4ppb

Cross-reaction rate:
SEM 100%
AMOZ less than 0.1%
AOZ less than 0.1%
AHD less than 0.1%

Recovery rate:
Tissue 75±25%
Honey 70±20%
Egg 95±25%

3. Nitrofuran SEM Diagnostic ELISA Kit Components

4. Samples preparation

Feed
1) Weigh 1± 0.05g of the homogenized sample, add9mL of the50% Ethanol absolute,then add5mL n-Hexane, fully shake for 3min;
2) Centrifuge at above 4000r/min at room temperature (20-25 ℃) for 10min;
3) Take5mL middle-layer water phase into another50mL clean centrifuge tube, add0.5mL 1 M HCI and 100µL 2-Nitrobenzaldehyde (C7H5NO3),fully shake for 2min;
4) Incubate at 70℃ by water bath for 20 minutes;
5) Add 5mL 0.1M K2HPO4, 0.4mL 1M NaOH and10mL ethyl acetate,fullyshake for 30s;
6) Centrifuge at above 4000r/min at room temperature (20-25 ℃) for 10min;
7) Transfer1mL oftheethyl acetate layer into a newcentrifugal tubeand evaporate to dryness by nitrogen or air at 50℃.
8) Dissolve the dry residues in2mL N-hexane, add 1mL of the diluted redissolving solution, mix properly for 30 seconds, centrifuge at above 4000 r/min at room temperature (20-25 ℃) for 10 min; Remove up-layerN-hexane phase;
9) Take 200µl down-layer clear liquid and 800µlof the diluted redissolving solution, mix it evenly;
10) Take 50 µLliquid for analysis.
Fold of dilution of the sample:100

5. Matters needing attention

1) If the room temperature is lower than 25℃ or the reagent temperature and the sample do not return to room temperature (20-25℃), the standard OD value will drop.
2) During the washing process, the drying of the microplate will be accompanied by the nonlinearity of the standard curve, and the reproducibility is not ideal; therefore, the next step should be performed immediately after washing.
3) Mix well, otherwise the reproducibility is poor.
4) The stop solution is 2 M sulfuric acid solution, avoid skin contact.
5) Do not use if the kit has expired. The use of diluted or adulterated reagents from the kit can result in changes in sensitivity and detection OD values. Do not replace reagents in different batches of kits.
6) Reseal unused microplates in ziplock bags. Standard solutions and colorless couplers are sensitive to light and cannot be directly exposed to light. Please store them in the dark.
7) Discard any coloring solution whose color indicates that the solution has degraded. A detection value of less than 0.5 for Standard Solution 1 (0 ppb) indicates degradation.
8) The optimal reaction temperature is 25℃, and the detection sensitivity and OD value will change if the temperature is too high or too low.

6. Storage and validity period

Storage: Store at 2-8°C, do not freeze.
Expiration date: 12 months; production date is on the box.
Note: If the microplate vacuum packaging leaks, it can still be used without affecting the test results, please use it with confidence.

Q1: When will it be shipped?
A1: We will ship the goods for you as soon as possible within 7 working days after receiving the payment. (In case of external factors such as the epidemic, the delivery may be delayed)

Q2: Does it support OEM/ODM?
A2: It can be supported, but the specific quantity needs to be more than 100,000 pieces, which is convenient for customized products.

Q3: How is your factory doing in terms of quality control?
A3: We have nationally certified ISO9001 and ISO13485. Our production process conforms to standard procedures to ensure optimum product quality.

Q4: How to provide after-sales service?
A4: We provide professional online technical after-sales service. We can provide you with one-on-one guidance via video, telephone, etc.

Q5: What is the payment method?
A5: We receive payment by T/T.

Q6: How to ship?
A6: Choose the best shipping method for you by getting quotes from our many cooperative carriers, and also ship according to your requirements.