Deoxynivalenol Diagnostic ELISA Kit for Feed 96 Wells/Kit Sensitivity 2ppb

1. Deoxynivalenol Diagnostic ELISA Kit Principle

This kit is based on the indirect competitive enzyme immunoassay for the detection of deoxynivalenol (DON). Conjugated antigens are pre-coated on microwell strips. Deoxynivalenol (DON) in the sample competes for anti-deoxynivalenol (DON) antibodies with the conjugated antigen pre-coated on the microwell stripes. After the enzyme conjugate is added, TMB substrate is added for staining. The optical density (OD) value of the sample was negatively correlated with deoxynivalenol (DON) in the sample. This value was compared to the standard curve and the deoxynivalenol (DON) residue was obtained.

2. Technical specifications

Sensitivity: 2ppb

Incubator temperature: 25℃

Incubator time: 30min~15min

Detection limit:

Cross-reaction rate:

Deoxynivalenol(DON) 100%

Recovery rate:

3. Deoxynivalenol Diagnostic ELISA Kit Components

4. Matters needing attention

1. The standard OD value will be low if the room temperature is lower than 25℃ or the reagent temperature and the sample has not returned to room temperature (20-25℃).
2. The microplate is dried during the washing process, which will be accompanied by non-linearity of the standard curve and poor reproducibility; therefore, proceed to the next step immediately after washing.
3. Mix well before adding any reagents.
4. The stop solution is 2M sulfuric acid solution, avoid contact with skin.
5. Do not use the kit beyond the expiration date. The use of diluted or adulterated reagents from the kit can result in changes in sensitivity and detection OD values. Do not replace reagents in different batches of kits.
6. Storage: Store at 2-8°C, do not freeze. Reseal unused microplates in self-sealing bags. Standard materials and colorless couplers are sensitive to light and cannot be directly exposed to light.
7. Discard any tinting solution whose color indicates that the solution has degraded. The detection value (450/630nm) of 0 standard solution (0 ppb) is less than 0.5 ((A450nm<0.5)) indicating its denaturation.
8. The optimal reaction temperature is 25℃. If the temperature is too high or too low, the detection sensitivity and OD value will change.

5. Storage and shelf life

Storage: Store at 2-8°C, do not freeze.
Expiration date: 12 months; production date is on the box.
Note: If the microplate vacuum packaging leaks, it can still be used without affecting the test results, so you can use it with confidence.

Q1: When will it be shipped?
A1: We will ship the goods for you as soon as possible within 7 working days after receiving the payment. (In case of external factors such as the epidemic, the delivery may be delayed)

Q2: Does it support OEM/ODM?
A2: It can be supported, but the specific quantity needs to be more than 100,000 pieces, which is convenient for customized products.

Q3: How is your factory doing in terms of quality control?
A3: We have nationally certified ISO9001 and ISO13485. Our production process conforms to standard procedures to ensure optimum product quality.

Q4: How to provide after-sales service?
A4: We provide professional online technical after-sales service. We can provide you with one-on-one guidance via video, telephone, etc.

Q5: What is the payment method?
A5: We receive payment by T/T.

Q6: How to ship?
A6: Choose the best shipping method for you by getting quotes from our many cooperative carriers, and also ship according to your requirements.