ISO9001 Diagnostic Porcine Veterinary Test Kit 192 Wells Per Kit PPV

Porcine Parvovirus Antibody Diagnostic ELISA Kit for Pig 192 Wells/Kit

1. Porcine Parvovirus Antibody Diagnostic ELISA Kit Usage

GreenSpring® Porcine Parvovirus (PPV) ELISA Kit is used for the qualitative detection of Porcine Parvovirus (PPV) IgG antibodies in pig serum; the assessment of the immune conditions of Porcine Parvovirus in pig farms and the epidemiological investigation of Porcine Parvovirus.

2. Principle

GreenSpring® Porcine Parvovirus (PPV) Antibody ELISA Detection Kit is made from antigen-coated microtiter plates (PPV antigen-coated) and other reagents. The principle of solid-phase ELISA was applied to PPV-Ab in serum, and then the enzyme conjugate was added to the microplate to specifically bind to the complex of coated antigen + PPV-Ab + enzyme-labeled anti-pig IgG. With TMB substrate, it produces a certain amount of color. The depth of color is related to the content of PPV-Ab. When the color value is greater than the critical value, the pigs are well inoculated or have natural infection.

3. Porcine Parvovirus Antibody Diagnostic ELISA Kit Components

4. User Notices and Warnings

1. This kit is for research use only.
2. Please read the manual carefully before use.
3. The experimental garbage should be sterilized by high pressure steam at 121℃ for 30 minutes, or treated with 5.0g/L sodium hypochlorite disinfectant for 30 minutes and then discarded.
4. Microplates removed from refrigeration should be equilibrated, dried at room temperature, and ready to be opened. Return unused MicroWell plates to dry foil bags and seal at 4 °C. Unused liquid reagents should be covered and stored together with other components at 2-8°C away from light.
5. Samples and reagents should be added using a micropipette and frequently demonstrated for accuracy.
6. When adding washing buffer, it should be topped up but not overflowed to avoid free enzyme or cross-contamination between wells.
7. The stop solution is corrosive, immediately rinse with plenty of water when it comes into contact with skin or clothes.

Specifications: 96 wells × 2.
Validity: 12 months.
Storage: 2~8℃, keep away from light.
Production date: on the outer packaging of the kit.

Epidemiological investigation

The dead fetus, piglets and excreta in the uterus of infected sows contain high titer of virus, while the live pigs produced by infected pigs may carry virus for a long time or even a lifetime. The infected boars are also the most dangerous source of infection of the disease. The virus can be isolated from the semen, spermatic cord, epididymis and gonads of boars. The boars can be transmitted to susceptible sows through breeding and spread the disease.
Polluted pig houses are the main storage of porcine parvovirus. The infected pigs can still be infected after the sick pigs are removed from the empty pen for 4.5 months and thoroughly cleaned before being put into the susceptible pigs. Contaminated food and pig saliva can be infectious for a long time.
Piglets, embryos and fetuses are vertically infected by infected sows; Boars, finishing pigs and sows are mainly infected by contaminated feed and environment through respiratory tract, reproductive tract or digestive tract; Most of the infection of primiparous sows occurred during breeding with infected boars; Mice can also spread the disease.
The disease is highly infectious. The introduction of the susceptible healthy pig herd - Danvirus can cause almost 100% infection in the pig herd within 3 months; The infected pigs remained seropositive for a long time. This disease mostly occurs in spring and summer festival or sow litter and mating season. When sows are infected in the early pregnancy, the mortality of embryos and fetal pigs can be as high as 80%~100%. Sows are most susceptible to infection in the first 30 to 40 days of pregnancy. Infection at different times of pregnancy will cause different symptoms, such as stillbirth, abortion, mummy, weak piglets and long term infertility of sows. The infection rate of virus is proportional to the age of animals.

Q1: When will it be shipped?
A1: We will ship the goods for you as soon as possible within 7 working days after receiving the payment. (In case of external factors such as the epidemic, the delivery may be delayed)

Q2: Does it support OEM/ODM?
A2: It can be supported, but the specific quantity needs to be more than 100,000 pieces, which is convenient for customized products.

Q3: How is your factory doing in terms of quality control?
A3: We have nationally certified ISO9001 and ISO13485. Our production process conforms to standard procedures to ensure optimum product quality.

Q4: How to provide after-sales service?
A4: We provide professional online technical after-sales service. We can provide you with one-on-one guidance via video, telephone, etc.

Q5: What is the payment method?
A5: We receive payment by T/T.

Q6: How to ship?
A6: Choose the best shipping method for you by getting quotes from our many cooperative carriers, and also ship according to your requirements.