Testing Principle
Amylase is a generic term for enzymes that hydrolyze starch and glycogen. Amylase generally acts on soluble starch, amylose, glycogen, etc., and cuts off the α-1,4-chain indiscriminately, and decomposes it into disaccharides and monosaccharides. Since monosaccharides are extracted during histochemical operations such as immobilization, dehydration, and embedding, the sugars that can be displayed on general tissue specimens are mainly polysaccharides, including glycogen, mucopolysaccharides, mucins, glycoproteins, glycolipid and so on. Therefore, to determine whether the red substance after PAS staining is glycogen, it is also necessary to carry out amylase digestion comparison experiments.
Stain Results
A red to reddish purple reaction in the cytoplasm of slice A without amylase digestion was positive for PAS staining. If slice B was negative after digestion, the positive substance of slice A was glycogen; if slice B was still positive after digestion and staining, it showed that the positive substance of the slice A was other polysaccharides.
Packaging Specification
Product Name | Ref # | Specification |
Amylase Solution | BA4187A | 20ml |
Amylase Solution | BA4187B | 100ml |