Specifications
Brand Name :
Biokey
Model Number :
BIK-Q-S011
Certification :
ISO 13485
Place of Origin :
Guangzhou China
MOQ :
We can produce liquid and lyophilized kits
Price :
USD
Supply Ability :
100,000 per day
Delivery Time :
Depending on order quantity
Packaging Details :
Carton package
Package :
48T/Box
Specimen :
Pig serum, feces, semen, viscera
Validity :
12 months
Storage :
Store away from light at -20±5℃
Applicable :
Micgene 242 / 244 、ABI QuantStudio5
CV :
≤5%
Description

Pig Pseudorabies Virus(gB gene) (PRV-gB) Detection Kit , DNA taq polymerase qPCR Probe kit

(PCR-Fluorescent Probe Method)

Intended use:

This kit is used for the diagnosis, monitoring and epidemiological investigation of Pseudorabies Virus(gB gene), and is suitable for the specific detection of PRV-GB gene in pig serum, feces, semen, viscera and other tissue samples.

Principle:

This kit use pig Pseudorabies Virus(gB gene) conservative design specific gene probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, thereby quenching groups out of the 3' end, Fluorescence was generated and detected by fluorescence PCR instrument to realize nucleic acid detection of PORcine PSEUDorabies virus gB gene in the completely closed reaction system.

Main components:

Component Specification
PCR reaction liquid 48 tubes
Positive control 1 tube
Negative control 1 tube
Note: components in different batches of kits should not be interchangeable.

Sample requirements:

1. Sample type:

Pig serum, feces, semen, viscera and other tissue samples

2. Sample collection and transportation:

Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.

3. Sample preservation:

It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -70℃.

The results of interpretation:

Results Ct Value
Positive Ct≤40
Negative No Ct value or Ct value=0
Gray area 40<Ct≤45

Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.

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Pig Pseudorabies Virus Porcine Detection Kit PRV GB DNA Qpcr Taqman Kit

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Brand Name :
Biokey
Model Number :
BIK-Q-S011
Certification :
ISO 13485
Place of Origin :
Guangzhou China
MOQ :
We can produce liquid and lyophilized kits
Price :
USD
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Pig Pseudorabies Virus Porcine Detection Kit PRV GB DNA Qpcr Taqman Kit

Guangzhou BioKey Healthy Technology Co.Ltd

Verified Supplier
3 Years
guangzhou
Since 2003
Business Type :
Manufacturer, Distributor/Wholesaler, Exporter, Seller
Total Annual :
5M-10M
Employee Number :
100~200
Certification Level :
Verified Supplier
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