This kit is applied for the specific detection of feline Herpes virus (FHV) in feline/cats' eye, mouth and nose secretion.
Principle
This kit uses RNA as a template and primers as a starting point to synthesize cDNA strands complementary to RNA template under the action of high-efficiency reverse transcriptase. Choose cat coronavirus gene conservative design specific probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, thereby quenching groups out of the 3' end, glow, Detection of cat/ feline herpes nucleic acid in a completely closed reaction system was realized by fluorescence PCR instrument.