Porcine reproductive and respiratory disorders syndrome(NA) Nucleic Acid Detection Kits (PCR-Fluorescent Probe Method)

Intended use:

This kit is used for the diagnosis, monitoring and epidemiological investigation of classical swine fever, and is suitable for the specific detection of porcine reproductive and respiratory syndrome virus (PRRSV) in oral swab, serum and viscera samples.

Principle:

This kit uses RNA as a template and primers as a starting point to synthesize cDNA strands complementary to RNA template under the action of high-efficiency reverse transcriptase. Selection of porcine reproductive and respiratory syndrome virus gene conservative design specific probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, thereby quenching groups out of the 3' end, The nucleic acid of PORcine reproductive and respiratory syndrome virus was detected by fluorescence PCR apparatus in a completely closed reaction system.

Reagent kits main components:

Product performance index:

1. Accuracy

The positive reference materials of testing enterprises were all positive.

2. Minimum detection limit:

The detection limit of this kit is 3 copies/μL.

3. Precision:

Coefficient of variation (CV,%) of intra-batch precision Ct value is ≤5%.

Sample requirements:

1. Sample collection and transportation:

Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.

2. Sample preservation:

It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -70℃.

The results of interpretation:

Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.