Specifications
Brand Name :
Biokey
Model Number :
BIK-Q-S008
Certification :
ISO 13485
Place of Origin :
Guangzhou China
MOQ :
We can produce liquid and lyophilized kits
Price :
USD
Supply Ability :
100,000 per day
Delivery Time :
Depending on order quantity
Packaging Details :
Carton package
Package :
24T/Box
Specimen :
Swab of tracheal secretion, lung tissue, etc
Validity :
12 months
Storage :
Store away from light at -20±5℃
Applicable :
Micgene 242 / 244 、ABI QuantStudio5
CV :
≤5%
Description

Actinobacillus pleuropneumoniae(APP) Nucleic Acid Detection Kits

(PCR-Fluorescent Probe Method)​

Intended use:

This kit is suitable for the detection of DNA of Actinobacillus pleuropneumoniae (APP) in tracheal secretions swabs, lung tissue samples, etc., for the auxiliary diagnosis of Actinobacillus pleuropneumoniae.

Principle:

This kit choose swine Actinobacillus pleuropneumoniae conservative design specific gene probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, thereby quenching groups out of the 3' end, Fluorescence was produced and detected by fluorescence PCR instrument to achieve the detection of actinobacillus pleuropneumoniae nucleic acid in the completely closed reaction system.

Main components:

Component Specification
PCR reaction liquid 24 tubes
Positive control 1 tube
Negative control 1 tube
Note: components in different batches of kits should not be interchangeable.

Sample requirements:

1. Sample type:

Swab of tracheal secretion, lung tissue, etc

2. Sample collection and transportation:

Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.

3. Sample preservation:

It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -70℃

The results of interpretation:

Results Ct Value
Positive Ct≤40
Negative No Ct value or Ct value=0
Gray area 40<Ct≤45

Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.

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Actinobacillus Pleuropneumoniae Porcine Test Kit Rt Pcr Fluorescence APP

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Brand Name :
Biokey
Model Number :
BIK-Q-S008
Certification :
ISO 13485
Place of Origin :
Guangzhou China
MOQ :
We can produce liquid and lyophilized kits
Price :
USD
Contact Supplier
Actinobacillus Pleuropneumoniae Porcine Test Kit Rt Pcr Fluorescence APP

Guangzhou BioKey Healthy Technology Co.Ltd

Verified Supplier
3 Years
guangzhou
Since 2003
Business Type :
Manufacturer, Distributor/Wholesaler, Exporter, Seller
Total Annual :
5M-10M
Employee Number :
100~200
Certification Level :
Verified Supplier
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