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Ureaplasma Parvum Ureaplasma Urealyticum Real Time PCR Detection Kit Lyophilized
Ureaplasma Parvum Ureaplasma Urealyticum Real Time PCR Detection Kit Lyophilized
Specifications
Brand Name :
Biokey
Model Number :
BIK-QL-H0022S1
Certification :
CE
Place of Origin :
Guangzhou China
MOQ :
We can produce liquid and lyophilized kits
Price :
USD
Payment Terms :
L/C, T/T, Western Union
Supply Ability :
100,000 per day
Delivery Time :
Depending on order quantity
Packaging Details :
Carton package
Package :
24 tests/kit
expiration date :
12 months
Storage :
-20±5℃
Applicable :
Micgene 244/244 IVD（BIO-KEY）
LoD :
1×1000 copies/ml
CV :
≤5%
Description
UP+UU real-time PCR detection kit (lyophilized)
--24 tests/kit


Intended use:

This kit, adopting Real-Time PCR-Fluorescence technology, is used for detection of Ureaplasma parvum and Ureaplasma urealyticum(UP+UU). It can be used for the laboratory diagnosis and monitoring of UP+UU infection. The test results are only for clinical reference, not for case confirmation or exclusion.

Principle:

The specific primers and specific fluorescent probes are designed for the nucleic acid conserved region of UP+UU. The PCR reaction solution and real-time fluorescent quantitative PCR detection technology is applied on the fluorescence quantitative PCR instrument to realize the rapid detection of UP+UU through the change of fluorescence signal. The detection system contains a human internal control (IC) gene, which is used to monitor whether the specimens and the process of nucleic acid extraction is qualified.

UP+UU product lyophilization pacakage:

Ureaplasma Parvum Ureaplasma Urealyticum Real Time PCR Detection Kit Lyophilized


Main components:

NO. Components

BIK-QL-H0022S1

1

PCR Enzyme Mix(Lyophilized)

24 tests/kit
2 Positive Control 100 μL/tube
3 Negative Control 100 μL/tube
4 RNase-free water 1mL/tube
5

Paraffin oil

1.5mL/tube
Note:Do not mix the components from different batches for detection. The Positive Control of UP+UU and internal control were constructed artificially, and they were not infectious.


Specimen requirements:

1. Specimen type

Male urethral secretions or female cervical secretions.

2. Specimen Preservation

The specimen can be stored for 3 months at -20±5℃ and for a long time below -70℃

PCR Amplification condition:


PCR amplification according to the following procedures:

Step Cycle number Temperature Time
1 1 95℃ 3min
2 40 95℃ 10s
60℃ 30s collect fluorescent

Instrument detection channel selection: FAM and HEX/VIC.

Quality control:

Negative Control: None of the FAM, VIC/HEX & Internal Control (ROX) channels have a Ct value or Ct ＞ 40;

Positive Control: FAM, VIC/HEX & Internal Control (ROX) channels Ct ≤ 30;

The above two items should be met simultaneously in one experiment. Otherwise, the experiment is invalid and should be repeated.


Data analysis and interpretation:

FAM

(UP)

VIC/HEX

(UU)

ROX

(IC)

Interpretation test

results

1

-

-

-

Result invalid,test again
2 + - +/- UP(+)
3 - + +/- UU(+)
4 + + +/- UP and UU(+)
5 - - + Negative(-)

NOTE: ‘+’ is positive, ‘-’ is negative.

Operation details please check from IFU!

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Ureaplasma Parvum Ureaplasma Urealyticum Real Time PCR Detection Kit Lyophilized

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Product Detail
Brand Name :
Biokey
Model Number :
BIK-QL-H0022S1
Certification :
CE
Place of Origin :
Guangzhou China
MOQ :
We can produce liquid and lyophilized kits
Price :
USD
Contact Supplier
Ureaplasma Parvum Ureaplasma Urealyticum Real Time PCR Detection Kit Lyophilized

Guangzhou BioKey Healthy Technology Co.Ltd

Verified Supplier
3 Years
guangzhou
Since 2003
Business Type :
Manufacturer, Distributor/Wholesaler, Exporter, Seller
Main Products :
RT qPCR Machine, Portable qPCR Machine, HPV PCR Kit
Total Annual :
5M-10M
Employee Number :
100~200
Certification Level :
Verified Supplier
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