Canine Babesiosis (B.C) Nucleic acid testing kit

(PCR-Fluorescent Probe Method)

Intended use:

This kit is suitable for the specific detection of Babesiosis (B.C) in canine EDTA whole blood and other samples.

Principle:

This kit uses the conserved gene fragment of Canine Babesiosis (B.C) to design a specific probe. The probe can specifically bind to a DNA template in the middle of the primer amplification region. During the PCR extension process, the exonuclease activity of the Taq enzyme will 5 The '-end fluorescent group is cleaved from the probe, making it free in the reaction system, thereby detaching from the 3'-end quenching group and emitting fluorescence, which is detected by a fluorescence PCR instrument to realize the detection of Canine Babesiosis (B.C) in a fully closed reaction system. Detection of Beth worm nucleic acid.

Main components:

Sample requirements:

1. Sample type

Canine EDTA whole blood etc.

2. Sample collection and transportation

Sample collection and transportation shall be carried out in accordance with NY/T 541. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.

3. Sample preservation

It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -70℃.

Method calibration:

PCR amplification and fluorescence Detection (PCR detection area)

Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:

FAM is selected for detection channels.

Positive judgment value or reference interval

Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.

Operation details please check from IFU!