Quinolones lateral flow assay diagnostics rapid test card tissue Aquatic products

Overview:

Quinolones (QNS) quick detection card is convenient, fast and sensitive, suitable for on-site mass sample detection. The test card contains antigen in the test area (T) and anti-ⅱ in the control area (C) of nitrocellulose membrane fixed in advance, and gold label antibody fixed on the binding pad. According to T area, C area color contrast to determine the result. Regardless of the presence of quinolones in the sample, a purplish red band appeared in region C.

Detection limit:

Store in dry place at 20±5℃, do not freeze, avoid direct sunlight.

This test card is valid for 12 months, and the production date is shown in the packaging box.

Materials required but not provided:

Detection Procedure:

1. Remove skin and fat from the samples to be tested and homogenize them with homogenizer;

2.Weigh 1.00±0.05 g homogenized sample into 10 mL centrifuge tube;

3.0.8ml extract A was added and vortexized for 1 min;

4. Add 4 mL extract B, vortex for 3 min, and centrifuge for 5 min at 4000 g;

5. Remove 500 µL supernatant into 5 mL centrifuge tube and blow dry with nitrogen (air) at 60℃;

6. Add 400 µL complex solution to the blow-dried centrifugal tube, shake well, and wait for inspection;

7. Take out the test card, place it flat on the table after opening, and carefully absorb 100 µL of supernatant from the centrifuge tube into the sample well with a pipette;

8. 5 min after adding samples, observe the color area and determine the result.

Result Judgment:

Negative: C line color, T line darker than C line color or consistent,The test results were negative.

Positive: C line is colored, T line is lighter than C line or T line is light green, and the test result is positive.

Invalid: Line C does not display color, regardless of whether line T displays color, the detection card is judged to be invalid.