Infectious Myonecrosis Virus Nucleic Acid Detection Kits

(PCR-Fluorescent Probe Method)

Intended use:

This kit is suitable for the diagnosis of Infectious Myonecrosis Virus (IMNV).

Principle:

This kit uses RNA as a template and primers as a starting point to synthesize cDNA strands complementary to RNA template under the action of high-efficiency reverse transcriptase. Choose peach virus gene conservative design specific probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, thereby quenching groups out of the 3' end, glow, The nucleic acid of taora virus was detected by fluorescence PCR in a completely closed reaction system.

Main components:

Sample requirements:

1. Sample type

Edible prawn, machijiming prawn, vannamei and other susceptible prawn tissues

2. Sample collection

About 0.1g tissue samples were taken from prawn samples according to different sizes or infection stages. Among them, whole individuals were taken from larvae and larvae, head and chest of eyes were removed from juveniles under 3CM, gill area was taken from larger juveniles, and gill filaments or heart or lymphatic organs were taken from adult shrimp.

3. Storage and transportation

Samples are transported in ice bags of 2℃~8℃, and repeated freezing and thawing are prohibited. Short-term storage at -20℃, -70℃ can be long-term storage.

Using Micgene 242/244/244IVD and ABI QuantStudio5:

procedures are set as below:

FAM was selected for detection channel.

The results of interpretation

Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 45 is considered as positive, and no Ct value or Ct value ≥45 is considered as negative.

Result analysis:
Automatic analysis using instrumentation

Quality control:
Negative control: No obvious amplification curve of FAM detection channel;
Positive control: FAM channel showed obvious amplification curve, Ct value ≤30;

The above conditions are met simultaneously in the same experiment; otherwise, the experiment is invalid and re-testing is required.

Operation details please check from IFU!