Enterocytozoon Acute hepatopancreatic necrosis syndrome Early mortality syndrome/Enterocytozoon hepatopenaei-18s(EMS/EHP-18S) Nuclear double nucleic acid test kit

Intended use:

This kit is suitable for the specific detection of Enterocytozoon hepatopenaei Acute hepatopancreatic necrosis syndrome Early mortality syndrome /Enterocytozoon hepatopenaei-18s (AHPND/EMS) and hepatoenteric cytiworm -18S (EHP-18S) in adult shrimp, prawn shrimp, shrimp seedlings, bait, processed aquatic products, etc.

Principle:

This kit is chosen prawn acute liver necrosis of pancreas disease and shrimp deeply cell worm - 18 s conservative design specific gene probe, the probe can with primer amplification area in the middle of a specific binding DNA template, in the process of PCR extension, the circumscribed Taq polymerase enzymes from the 5 'end of fluorescent groups on the probe will be cut down, make it free in the reaction system, Thus, the 3 '-end quenchable group was separated and fluorescence was emitted, which was detected by fluorescence PCR instrument to realize the detection of acute hepatopancreas necrosis and hepatoenteric cytiworm -18S nucleic acid of shrimp in the completely closed reaction system.

Main components:

Sample requirements:

1. Sample type

Adult shrimp, shrimp, shrimp seedlings, bait, processed aquatic products and other samples

2. Sample collection and transportation

Samples shall be collected and transported in accordance with SC/T 7103. Samples shall be transported to the laboratory as soon as possible under refrigerated conditions to avoid repeated freeze-thaw.

3. Sample preservation

It can be stored for 24 hours at 2℃~8℃, and can be stored for a long time at -70℃.

Using Micgene 242/244/244IVD and ABI QuantStudio5:

PCR amplification and fluorescence Detection (PCR detection area)

Put each reaction tube into the PCR instrument and perform PCR amplification according to the following procedures:

FAM and VIC/HEX are selected for detection channels.

Result interpretation:

Note: "+" means positive, "-" means negative

The results of interpretation

Note: The result is judged to be grey area, which needs to be rechecked. If the recheck result is Ct value < 40 is considered as

Operation details please check from IFU!