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Digestive Bacteria Infection NAT Fluorescent Quantitative H Pylori Hp PCR Test Kit
Digestive Bacteria Infection NAT Fluorescent Quantitative H Pylori Hp PCR Test Kit
Digestive Bacteria Infection NAT Fluorescent Quantitative H Pylori Hp PCR Test Kit
Digestive Bacteria Infection NAT Fluorescent Quantitative H Pylori Hp PCR Test Kit
Specifications
Brand Name :
Biokey
Model Number :
BIK-QL-H008
Certification :
ISO 13485,CE
Place of Origin :
Guangzhou China
MOQ :
We can produce liquid and lyophilized kits
Price :
USD
Payment Terms :
L/C, T/T, Western Union
Supply Ability :
100,000 per day
Delivery Time :
Depending on order quantity
Packaging Details :
Carton package
Package :
96 T/kit (carton)
Specimen :
Biopsy samples of human gastric mucosa, saliva, dental plaque, feces, etc.
Specimen preservation :
be stored for 3 months at -20±5℃ and for a long time below -70℃
Applicable :
Micgene 244/244 IVD(BIO-KEY)
Detection limit :
500 copies/mL
CV :
≤5%
Description

Fluorescent quantitative Hp PCR kit Helicobacter pylori (H.p) Real Time PCR Detection Kit (Lyophilized)

Intended use:

This kit, adopting Real-Time PCR-Fluorescence technology, is used for detection of H.p. It can be used for the laboratory diagnosis and monitoring of the Hp infection. The test results are only for clinical reference, not for case confirmation or exclusion.

Test Principle:


The specific primers and specific fluorescent probes are designed for the nucleic acid conserved region of Hp. The PCR reaction solution and real-time fluorescent quantitative PCR detection technology is applied on the fluorescence quantitative PCR instrument to realize the rapid detection of Hp through the change of fluorescence signal. The detection system contains a human internal control (IC) gene, which is used to monitor whether the specimens and the process of nucleic acid extraction is qualified.


Product components:

No. Components BIK-QL-H008
1 PCR Enzyme Mix (Lyophilized) 96 tests/bottle
2 Hp Primer-Probe Mix 100 μL/vial
3 Enzyme Mix Buffer (5×) 400 μL/vial
4 Positive Control 100 μL/tube
5 Negative Control 100 μL/tube
6 RNase-free water 1mL/tube
7 Paraffin oil 1.5mL/tube*2

Note: Do not mix the components from different batches for detection. The Positive Control of Hp and internal control were constructed artificially, and they were not infectious.

Compatible instruments:

The diagnostic kit is applicable to PCR instrument with multi-color fluorescence channel FAM,VIC/HEX and ROX, including Quant Studio5 (ABI), Micgene 244/244 IVD(BIO-KEY).

Reaction Mix Preparation:

Refer to one of the table below to prepare the reaction mix:

1×volume required
Resuspended master mix 14 μL
Hp Primer & probe 1 μL
Total volume 15 μL

※Multiply the numbers according to the number of tests.

PCR amplification condition:

PCR amplification according to the following procedure:

Step Cycle Temperature Time
1 1 94℃ 3 min

2

40

 94℃ 10 s
60℃

30 s

(Collection of fluorescence)

Select the detection channels as following:

a) Select FAM channels to detect Hp.

b) Select HEX/VIC channel to detect internal control.

Product picture:

Digestive Bacteria Infection NAT Fluorescent Quantitative H Pylori Hp PCR Test Kit

Operation details please check from IFU

Product tags

h pylori testing kit

,

h pylori home test kit

,

what is h pylori test
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Digestive Bacteria Infection NAT Fluorescent Quantitative H Pylori Hp PCR Test Kit

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Product Detail
Brand Name :
Biokey
Model Number :
BIK-QL-H008
Certification :
ISO 13485,CE
Place of Origin :
Guangzhou China
MOQ :
We can produce liquid and lyophilized kits
Price :
USD
Contact Supplier
Digestive Bacteria Infection NAT Fluorescent Quantitative H Pylori Hp PCR Test Kit
Digestive Bacteria Infection NAT Fluorescent Quantitative H Pylori Hp PCR Test Kit

Guangzhou BioKey Healthy Technology Co.Ltd

Verified Supplier
3 Years
guangzhou
Since 2003
Business Type :
Manufacturer, Distributor/Wholesaler, Exporter, Seller
Main Products :
RT qPCR Machine, Portable qPCR Machine, HPV PCR Kit
Total Annual :
5M-10M
Employee Number :
100~200
Certification Level :
Verified Supplier
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