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Fast NAT Amplification Detection Enterovirus 71 EV71 RT PCR Test Kit Lyophilized
Fast NAT Amplification Detection Enterovirus 71 EV71 RT PCR Test Kit Lyophilized
Fast NAT Amplification Detection Enterovirus 71 EV71 RT PCR Test Kit Lyophilized
Fast NAT Amplification Detection Enterovirus 71 EV71 RT PCR Test Kit Lyophilized
Specifications
Brand Name :
Biokey
Model Number :
BIK-QL-H003 / BIK-QL-H003S
Certification :
ISO 13485,CE
Place of Origin :
Guangzhou China
MOQ :
We can produce liquid and lyophilized kits
Price :
USD
Payment Terms :
L/C, T/T, Western Union
Supply Ability :
100,000 per day
Delivery Time :
Depending on order quantity
Packaging Details :
Carton package
Package :
96 T/kit (carton) / 48 T/kit (single tube)
Specimen :
Human throat swabs, herpes fluid samples, stool samples, and anal swab samples.
Specimen preservation :
be stored for 3 months at -20±5℃ and for a long time below -70℃
Applicable :
Micgene 244/244 IVD(BIO-KEY)
Detection limit :
500 copies/mL
CV :
≤5%
Description

Fast Amplification PCR Test Kit Enterovirus 71 (EV71) RT-PCR Detection Kit (Lyophilized)

Intended use:

By using real time PCR systems, Enterovirus 71 real time PCR kit is used for the in vitro qualitative detection of Enterovirus 7. It can be used for the laboratory diagnosis and monitoring of the EV71 infection, which often causes hand-foot and mouth disease (HFMD). The test results are only for clinical reference, not for case confirmation or exclusion.

Test Principle:


The specific primers and specific fluorescent probes are designed for the nucleic acid conserved region of EV71. The RT-PCR reaction solution and real-time fluorescent quantitative PCR detection technology is applied on the fluorescence quantitative PCR instrument for realizes the rapid detection of EV71 through the change of fluorescence signal. The detection system contains a human internal control (IC) gene, which is used to monitor whether the specimens and the process of nucleic acid extraction is qualified.


Product components:

No. Components BIK-QL-H003 BIK-QL-H003S
1

PCR Enzyme Mix

(Lyophilized)

 96 tests/bottle 48 tests/kit
2 EV71 Primer-Probe Mix 100 μL/vial /
3 Enzyme Mix Buffer (5×) 400 μL/vial /
4 Positive Control 100 μL/tube 100 μL/tube
5 Negative Control 100 μL/tube 100 μL/tube
6 RNase-free water 1mL/tube 1mL/tube
7 Paraffin oil 1.5mL/tube*2 1.5mL/tube

Note: Do not mix the components from different batches for detection. The Positive Control of EV71 and internal control were constructed artificially, and they were not infectious.

Compatible instruments:

The diagnostic kit is applicable to PCR instrument with multi-color fluorescence channel FAM,VIC/HEX and ROX, including Quant Studio5 (ABI), Micgene 244/244 IVD(BIO-KEY).

Reaction Mix Preparation:

Refer to one of the table below to prepare the reaction mix:

1×volume required
Resuspended master mix 14 μL
EV71 Primer & probe 1 μL
Total volume 15 μL

※Multiply the numbers according to the number of tests.

PCR amplification condition:

PCR amplification according to the following procedure:

Step Cycle Temperature Time
1 1 50℃ 10 min
2 1 94℃ 3 min

3

40

 94℃ 10 s
60℃

30 s

(Collection of fluorescence)


Select the detection channels as following:

a) Select FAM channels to detect EV71.

b) Select HEX/VIC channel to detect internal control.

Reaction Mix Preparation:

Refer to one of the table below to prepare the reaction mix:

1×volume required
Resuspended master mix 14 μL
EV71 Primer & probe 1 μL
Total volume 15 μL

※Multiply the numbers according to the number of tests.

Product picture:

Fast NAT Amplification Detection Enterovirus 71 EV71 RT PCR Test Kit Lyophilized

Fast NAT Amplification Detection Enterovirus 71 EV71 RT PCR Test Kit Lyophilized

Operation details please check from IFU

Product tags

test optocoupler

,

rs485 tester

,

hepa filter dop test
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Fast NAT Amplification Detection Enterovirus 71 EV71 RT PCR Test Kit Lyophilized

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Product Detail
Brand Name :
Biokey
Model Number :
BIK-QL-H003 / BIK-QL-H003S
Certification :
ISO 13485,CE
Place of Origin :
Guangzhou China
MOQ :
We can produce liquid and lyophilized kits
Price :
USD
Contact Supplier
Fast NAT Amplification Detection Enterovirus 71 EV71 RT PCR Test Kit Lyophilized
Fast NAT Amplification Detection Enterovirus 71 EV71 RT PCR Test Kit Lyophilized

Guangzhou BioKey Healthy Technology Co.Ltd

Verified Supplier
3 Years
guangzhou
Since 2003
Business Type :
Manufacturer, Distributor/Wholesaler, Exporter, Seller
Main Products :
RT qPCR Machine, Portable qPCR Machine, HPV PCR Kit
Total Annual :
5M-10M
Employee Number :
100~200
Certification Level :
Verified Supplier
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