SUMMARY

Mumps Virus (Mu) is a pathogen of mumps, belonging to the Paramyxoviridae family. It was first discovered in 1935 and proved to be the main cause of mumps. The popularity of MuV is peaked in winter and spring, and is limited to humans. MuV is the only source of infection for infected people. The main routes of transmission are saliva, saliva-contaminated foods and articles, and airborne droplets. Children and adolescents are susceptible to mumps. Clinical take parotid gland enlargement as main characteristic, common complication has virus meningitis and encephalitis, orchitis, epididymis inflammation, still have ovarian inflammation, pancreatitis, myocarditis to wait in addition, it is the male infertile disease and child acquired sex deaf most common reason, serious person can bring about disable or death. Mumps virus IgM antibody appeared shortly after the onset (2 ~ 3 days), and reached a peak within 2 weeks, and then decreased gradually, which could be kept for several months, and was an important basis for early diagnosis of MuV infection.At present, the main methods to diagnose MuV infection in clinic or laboratory include virus isolation and culture, serological detection (complement binding test, neutralization test, etc.), immunological detection (enzyme-linked immunoassay, indirect immunofluorescence detection, etc.) and molecular biological methods such as rt-pcr.

SAMPLE REQUIREMENT

1. Serum samples are collected by intravenous method according to the conventional method; plasma sample processing method: 1% heparin solution 100 μL anticoagulation 5-10 mL blood, 3.8% sodium citrate solution and blood anticoagulation in a ratio of 1:9, 15% 0.04 mL of disodium edetate (EDTA) solution was anticoagulated with 5 mL of blood.

2. Serum or plasma samples can be stored within 5 days after collection, and can be stored at 4 °C. For samples larger than 5 days, samples should be stored at -20 °C. The number of freeze-thaw cycles should not exceed 3 times. .

3. Whole blood samples are recommended to be tested within 3 days. The sample is stored at 2~8 °C. Do not freeze.

4. Hemolysis sample test results are invalid.