SUMMARY

Human parvovirus (HPV B19) is the only pathogen of the genus Parvovirus that is pathogenic to humans. It belongs to the family of Parvoviridae and genus of erythropoiesis. Parvoviruses are mainly transmitted through the respiratory and bloodstream, with an incubation period of 6 to 8 days. For people with normal immunity, it does not cause symptoms or very mild symptoms, and it has lifelong immunity. Infection in patients with immunodeficiency or blood diseases can cause complications such as arthritis and vasculitis or secondary infections; patients with hemolytic anemia will develop temporary aplastic anemia; chronic inhibition of erythropoiesis presents symptoms of chronic anemia; early pregnancy can cause miscarriage, fetal malformation or stillbirth; in addition, parvovirus B19 is also the cause of infectious rash in children. After HPV B19 infects the body, symptoms usually appear within 3 days. At this time, 90% of patients can detect IgM antibodies; IgG antibodies appear after about 2 weeks; IgM usually disappears 2-3 months after the disease. There are many methods for detecting B19 infection, such as immunoelectron microscopy and receptor-mediated hemagglutination test, but the commonly used detection methods in the laboratory are still nucleic acid detection and serological detection.

SAMPLE REQUIREMENT

1. Serum samples are collected by intravenous method according to the conventional method; plasma sample processing method: 1% heparin solution 100 μL anticoagulation 5-10 mL blood, 3.8% sodium citrate solution and blood anticoagulation in a ratio of 1:9, 15% 0.04 mL of disodium edetate (EDTA) solution was anticoagulated with 5 mL of blood.

2. Serum or plasma samples can be stored within 5 days after collection, and can be stored at 4 °C. For samples larger than 5 days, samples should be stored at -20 °C. The number of freeze-thaw cycles should not exceed 3 times. .

3. Whole blood samples are recommended to be tested within 3 days. The sample is stored at 2~8 °C. Do not freeze.

4. Hemolysis sample test results are invalid.