For Laboratory Or Hospital High Accuracy Follicle-Stimulating Hormone Elisa Test Kit

INTENDED USE

The FT3 assay is an enzyme-linked immunosorbent assay (ELISA) for the in vitro quantitative determination of free triiodothyronine (FT3) in human serum. The assay is useful in the diagnosis and treatment of thyroid disorders. For professional use only.

Test principle

Test principle Competition principle. Total duration of assay: 80 minutes

• Sample, T3 derivant coated microwells and enzyme labeled Anti-T3 are combined.

• During the incubation, T3 derivant coated on microwells and FT3 present in the sample compete for binding to the enzyme labeled antibodies.

• After washing, a complex is generated between the solid phase and enzyme-linked antibodies by immunological reactions.

• Substrate solution is then added and catalyzed by this complex, resulting in a chromogenic reaction. The resulting chromogenic reaction is measured as absorbance.

• The color intensity is inversely proportional to the amount of FT3 in the sample.

Materials provided

• FT3 Coated Microplate - symbol T3 derivant.

• FT3 Calibrators - symbols FT3 PLATE FT3 CAL A-F 8 x 12 strips, 96 wells, pre-coated with 6 vials, 1 mL each, ready to use; Concentrations: 0(A), 2(B), 5(C), 10(D), 20(E) and 50(F) pmol/L.

• FT3 Enzyme Conjugate - symbol FT3 CONJ 1 vial, 6.0 mL of HRP (horseradish peroxidase) labeled sheep monoclonal Anti-T3 in Tris-NaCl buffer containing BSA (bovine serum albumin). Contains 0.2% ProClin300 preservative.

• Substrate - symbol TMB. SUBSTRATE STOP 1 vial, 11mL, ready to use, (tetramethylbenzidine)

• Stop Solution - symbol 1 vial, 6.0 mL of 1 mol/L sulfuric acid.

• Wash Solution Concentrate - symbol PBS-Tween wash solution.

• IFU - 1 copy.

• Plate Lid: 1 piece.