Respiratory V (lung infection) - Cat Nucleic Acid Test Kit (Dry) (FHV, FCV, Mycoplasma, Chlamydia, B. bronchiseptica)
Attribute | Value |
---|---|
Product Name | PCR amplification solution |
Product Type | DNA amplification reagent |
Enzyme Type | Taq DNA Polymerase |
Concentration | 10X |
Reaction Conditions | Standard |
Quality Control | Endonuclease and Exonuclease Activity Tested |
Package Size | 48 Tests/kit |
PCR Time | 45min |
Storage Temperature | 2-8°C |
Catalog Number | K1081 |
Template DNA | Not Included |
Storage Condition | 2-8℃ Away From Light |
Product Description | Polymerase chain reaction reagent kit for 48 tests |
Testing Principle】 The test kit uses nucleic acid extraction reagents to extract the nucleic acid (DNA/RNA) from the sample. Under the action of a high-efficiency reverse transcriptase, cDNA complementary to the RNA template is synthesized in a one-step reaction using RNA as the template. Under the action of Taq enzyme, the copy number of the specific target fragment is amplified through cycles of high-temperature denaturation, annealing at a moderate temperature, and extension using DNA as the template. The fluorescence-labeled specific probe hybridizes with the amplified target fragment, and the 5'→3' exonuclease activity of Taq polymerase separates the reporting group and quencher group of the fluorescence probe, emitting a specific f luorescence signal. The specific fluorescence signal is detected using a f luorescence PCR instrument, and the result is determined based on the Ct value of the sample and the formation of the amplification curve.
1. Shelf life: 18 months.
2. Production date and expiration date are on the package
This test kit is compatible with real-time quantitative f luorescence PCR instrument.