Specifications
Model Number :
In-Ch0032
Place of Origin :
CHINA
MOQ :
1 Box
Price :
Negotiable
Payment Terms :
L/C, Western Union, T/T
Supply Ability :
1000kits/week
Delivery Time :
5-8 working days
Packaging Details :
96wells/box or 48wells/box
Short names :
IL-1
Method :
Sandwich
Cat. No. :
In-Ch0032
Gene names :
IL1
Standard :
135pg/ml
Package Size :
96 tests/Kit
Brand Name :
BIOVANTION
Description

Chicken Interleukin 1,IL-1 ELISA Kit; Research Use Elisa Kit

  • For research use only.
  • For the quantitative in vitro determination of Chicken Interleukin 1(IL- 1) concentrations in Chicken serum, plasma, culture media or any biological fluid.
  • Expiration date:six months .
  • Storage: 2-8℃.
  • Package Size :96 tests/Kit

Principle

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to IL-1 Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for IL-1 is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain IL-1 and HRP conjugated IL-1 antibody will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of IL-1. You can calculate the concentration of IL-1 in the samples by comparing the OD of the samples to the standard curve.

Materials provided with the kit

Materials provided with the kit 48 determinations 96 determinations Storage
1 User manual 1 1 R.T.
2 Closure plate membrane 2 2 R.T.
3 Sealed bags 1 1 R.T.
4 Microelisa stripplate 1 1 2-8℃
5 Standard:135pg/ml 0.5ml×1 bottle 0.5ml×1 bottle 2-8℃
6 Standard diluent 1.5ml×1 bottle 1.5ml×1 bottle 2-8℃
7 HRP-Conjugate reagent 3ml×1 bottle 6ml×1 bottle 2-8℃
8 Sample diluent 3ml×1 bottle 6ml×1 bottle 2-8℃
9 Chromogen Solution A 3ml×1 bottle 6ml×1 bottle 2-8℃
10 Chromogen Solution B 3ml×1 bottle 6ml×1 bottle 2-8℃
11 Stop Solution 3ml×1 bottle 6ml×1 bottle 2-8℃
12 Wash solution 20ml (20X)×1bottle 20ml (30X)×1bottle 2-8℃

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IL-1B were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IL-1B were tested on 3 different plates, 8 replicates in each plate.

CV(%) = SD/meanX100

Intra-Assay: CV<10%

Inter-Assay: CV<12%

Assay range

1 pg/ml -100 pg/ml

Sensitivity:

0.1 pg/ml

96 Wells IL1 Research RUO Test Kit Chicken Interleukin 1 ELISA Kit96 Wells IL1 Research RUO Test Kit Chicken Interleukin 1 ELISA Kit96 Wells IL1 Research RUO Test Kit Chicken Interleukin 1 ELISA Kit

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96 Wells IL1 Research RUO Test Kit Chicken Interleukin 1 ELISA Kit

Ask Latest Price
Model Number :
In-Ch0032
Place of Origin :
CHINA
MOQ :
1 Box
Price :
Negotiable
Payment Terms :
L/C, Western Union, T/T
Supply Ability :
1000kits/week
Contact Supplier
96 Wells IL1 Research RUO Test Kit Chicken Interleukin 1 ELISA Kit
96 Wells IL1 Research RUO Test Kit Chicken Interleukin 1 ELISA Kit

Biovantion Inc.

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4 Years
beijing, beijing
Business Type :
Manufacturer, Importer, Exporter, Trading Company, Seller
Certification Level :
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