HBc-IgM ELISA Kit
Catalog No.: BE106A

1. SUMMARY

Hepatitis B is an infectious illness caused by hepatitis B virus (HBV) which is an enveloped, double-stranded DNA virus belonging to the Hepadnaviridae family and is recognized as the major cause of blood transmitted hepatitis together with hepatitis C virus (HCV).

HBV is excreted in body fluids such as semen, saliva, blood and urine in persons with acute or chronic infection. The hepatitis B virus is known as a blood-borne virus because it is transmitted from one person to another via blood or fluids contaminated with blood. Transmission of hepatitis B virus results from exposure to infectious blood or body fluids.When HBV invades the body it causes liver damage through induction of auto-immunity.

Three immunity has been found, surface antigen (HBsAg)/HBsAb, core antigen (HBcAg)/HBcAb and e antigen(HBeAg)/HBeAb. As it is difficult to detect the core antigen in the serum, the other five are been done to diagnosing HBV.

2. MATERIALS PROVIDED

1. Microplate 1 block (96wells)
2. Negative Control 1 ml ×1
3. Positive Control 1 ml ×1
4. Conjugate 6 ml ×1
5. Substrate Solution A 6 ml ×1
6. Substrate Solution B 6 ml ×1
7. 20×Wash Buffer 40 ml ×1
8. Stop Solution 6 ml ×1
9. Plate Cover 3 piece
10. Insert 1 copy

3. TEST PROCEDURE

1. Dilute samples: Use the Normal Saline(0.9% NaCl) to dilute the samples(The samples must be diluted 1:999 before use.). The Positive Control and Negative Control have no use for dilution.
2. Add samples:Open the foil pouch and remove the microplate. Dispensing 50μl of diluted samples , Positive Control and Negative Control to each well except the blank well. Gently vibrating the microplate. Cover and incubate for 30 minutes at 37℃.
3. Wash the plate:Dilute 1 volume of wash buffer concentrate with 19 volumes of distilled water, mix well. Remove the solutions from all the wells. Fill the wells with the diluted wash solution (at least 20 seconds to soak) then remove the diluted wash buffer from the wells. Repeat the procedure for 5 times. Make sure that the rest volume is minimal, by blotting dry by tapping plate onto absorbent paper.
4. Add conjugate:Add the 50μl of conjugate to each well (except the blank well). Cover and incubate for 30 minutes at 37℃.
5. Wash the plate:Repeat the wash procedure as in step 2.
6. Add 50μl of Substrate Solution A and 50μl of Substrate Solution B to each well, mix well. Cover and incubate for 10 minutes at 37℃.
7. Stop reaction:Add 50μl stop solution to each well, mix well.
8. Read the absorbance at 450 nm. If a dual wavelength measurement is used, the reference wavelength should be selected from 620nm to 690nm.
9.