Shanghai Korain Biotech Co., Ltd

Shanghai Korain Biotech Co Ltd

OEM is also available by us. We can produce as any request from customer.

Korain’s reagents are supported by superior processional team and a quality management system that is certified for CE and ISO. Our product development program for use in a variety of applications including:

• ELISA and immunohistochemistry
• Immunoprecipitation
• Immunohistochemistry
• Immunofluorescence microscopy
• Immunohistochemistry
• Quantitative Multiplexing

Packing and Shipment

Custom Package

• different types for you to choose
• Ice packc and polyfoam material to wrap

Ship by DHL/FedEX at our discounted rate

• Order be shipped within 24 hrs
• Bulk order shipped within 5 business days

Techinical Laboratory Clean Breeding Environment

ELISA Kit Developing Service

Sandwich/Competitive ELISA kit

Need to be provided by customers:

1. Target Proteinor small molecule compound; Possible matched antibody pairs (poly/mono antibody)
2. Control kit
3. Relevant technical literatures

Antibody or Antibody pair can be specifically provided as customer's request.

Developing charges: 1650USD (Not including the antibody fees)

Project time：15-20 days
Developing Results:
What we provide:
10 Completed ELISA kits to customer.
Standard curve report and determination data report.
Over 10 ELISA kits: 99USD/kit (96T)

Antibody Custom Production

1. Mouse Monoclonal Antibody ProductionProvided by customers：

1) Recombinant purification protein or natural purified protein 5mg(protein purity should be higher than 80%)

2) Synthetic polypeptide antigen (5mg). Or provide the seqres of polypeptide to us, we will synthesizing/coupling polypeptide.

3) Inactivated bacteria or viruses, or bacteria, virus proteins (notes: must not be contagious, and harmless to human).

4) Provide the gene sequences of functional proteins to us only, then we could also recombine& express the protein for customer.

Producing procedure
1. Vaccinating 5 BALB/ c strain mice with standard immune procedure. The antibody titers in serum will be determined by ELISA.

2. After the antibody titers are achieved appropriate values, the mouse spleen cells were fused to form hybridoma. Then determining the supernatant of growing hybridoma cell by ELISA.

3. Subcloning the hybridoma cell lines by the method of finite dilution, finally provide 2 hybridoma cell lines to the customer.

4. The Preparation of BALB/ c strain mice Ascites, antibody purification.

The project completion time: 4-5 months

2.Rabbit Polyclonal Antibody Production

Provided by customers：
1) Purification protein or natural purified protein 10mg (protein purity should be higher than 80%).

2) Prepare polyclonal antibody with polypeptide antigen (10mg). Or provide the seqres of polypeptide to us, we will synthesizing/coupling polypeptide.

3) Inactivated bacteria or viruses, or bacteria, virus proteins (notes: must not be contagious, and harmless to human).

4) Provide the gene sequences of functional proteins to us only, then we could also recombine& express the protein for customer.

Producing procedure：

Polypeptide or Protein Antigen/ Mycoprotein, etc. Immunizing 1-2 New Zealand rabbits (or 3 rats); determine the titers by collecting (rabbits) auricular arterial blood, serum were collected by bleeding of carotid artery; determine the titers by collecting (rats) tail veins blood, collected serums from heart.

Production result

Plan 1: Provide 10ml serum, titers >1: 10000. Including the report of titers value which were determined by indirect ELISA.
Plan 2: Povide 10 mg (Rabbit) Polyclonal antibody which was extracted by precipitation with ammonium sulfate, 2 mg (Rat) Polyclonal antibody which was extracted by precipitation with ammonium sulfate, antibody titers(1mg/ml) > 1: 5000; Including the report titers value which were determined by indirect ELISA.
Plan 3: According to the requirement, collecting serum by sustaining immunization.

The project completion time: 4-5months.

Antigen Production sevice

Our technical team consist of professional staff and expert.

Shanghai Korain begun at 2010, and then Bioassay Technology Laboratory (BT Lab) comes out as a brand under korain; as a leading brand of Korainbio focused on helping researchers to optimize life science work and serves as a provider of test and developing services, including elisa tests, WB test and antigen development. Founding in 2010, our strategic focus has been on the development of enabling technologies in the research, development, manufacture and marketing of innovative immuno products and services based on molecular technologies.
Now BT Lab is growing stronger.

What We Treasure
Creative & Integrity

What We Aim to

• Optimize your life science research with our tools
• Build a friendship with every single customer
• Devoted ourselves to providing researchers with professional support

What We Provide:

• Product datasheet, product image, MSDS, publican references
• Order by emailor phone.
• Quality Guarantee with ISO and CE certificate
• Within 36 hours to your inquiry
• Support by professional team who are specializes in life science.
• Delivery to more than 100 countries
• Special packaging according your request