High efficiency Rabbit anti GST-Tag pAb

Cat.No. BT8005V Size: 10ug Datasheet Request

Product Information

Clonallity: Monoclonal

Applications: WB, IP, FC, ELISA

Source: Rabbit

Immunogen: Recombinant Protein

Concentration: 1mg/ml

Observed molecular weight: 26 kDa

Formulation

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol

Purification method:

Antigen affinity purification

Species specificity:

recombinant protein, human

Positive WB detected in:

Recombinant protein

Positive FC detected in:

HeLa cells

Synonyms:

Glutathione S Transferase mu,glutathione-S-transferase, Glutathione S Transferase, Glutathione S transferase Mu 1, GST 26, GST class mu 1, GST1, GSTM1, GSTM1 1, GSTM1a 1a, GSTM1b 1b, GTH4, GTM1, H B, HB subunit 4, MGC26563, MU 1, MU, SJ26 antigen, SjGST, GST-tag

Recommended Dilution:

WB : 1:1000-1:4000

Not yet tested in other applications

Storage:

PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC.Aliquoting is unnecessary for -20oC storage.

Background

GST(Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. Antibodies to GST are useful for checking protein expression both in plaques and on western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody can be used to detect the GST tagged protein. This antibody can be used as Rabbit IgG control in FC.

Western blot analysis of GST-tagged protein using GST-tag antibody at different dilution. Each lane was loaded with 5 ug protein.

Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.

Lysates/proteins: 25ug per lane.

Blocking buffer: 3% nonfat dry milk in TBST.