Bovine Fetuin A ELISA Kit with High Spesificity and Sensitivity
Cat.No E2018Bo
Reagent Provided
Components | Quantity |
Standard Solution (1920mg/L) |
0.5ml x1 |
Pre-coated ELISA Plate | 12 * 8 well strips x1 |
Standard Diluent | 3ml x1 |
Streptavidin-HRP | 6ml x1 |
Stop Solution | 6ml x1 |
Substrate Solution A | 6ml x1 |
Substrate Solution B | 6ml x1 |
Wash Buffer Concentrate (30x) | 20ml x1 |
Biotinylated Bovine FETU-A Antibody | 1ml x1 |
User Instruction | 1 |
Plate Sealer | 2 pics |
Zipper bag | 1 pic |
Reagent Preparation
All reagents should be brought to room temperature before use.
Standard Reconstitute the 120μl of the standard (1920mg/L) with 120μl of standard diluent to generate a 960mg/L standard stock solution. Allow the standard to sit for 15 mins with gentle agitation prior to making dilutions. Prepare duplicate standard points by serially diluting the standard stock solution (960mg/L) 1:2 with standard diluent to produce 480mg/L, 240mg/L, 120mg/L and 60mg/L solutions. Standard diluent serves as the zero standard(0 mg/L). Any remaining solution should be frozen at -20℃ and used within one month. Dilution of standard solutions suggested are as follows:
960mg/L | Standard No.5 | 120μl Original Standard + 120μl Standard Diluent |
480mg/L | Standard No.4 | 120μl Standard No.5 + 120μl Standard Diluent |
240mg/L | Standard No.3 | 120μl Standard No.4 + 120μl Standard Diluent |
120mg/L | Standard No.2 | 120μl Standard No.3 + 120μl Standard Diluent |
60mg/L | Standard No.1 | 120μl Standard No.2 + 120μl Standard Diluent |
Standard Concentration | Standard No.5 | Standard No.4 | Standard No.3 | Standard No.2 | Standard No.1 |
1920mg/L | 960mg/L | 480mg/L | 240mg/L | 120mg/L | 60mg/L |