Have you ever experienced the need for experiments involving fungi or cells that require ventilation, but struggled to find suitable culture media during tissue culture propagation, discovered poor air circulation leading to unsuccessful growth, and then faced bacterial contamination after making air holes in tissue culture bags or bottles. So, how can we prevent such situations? Today, we will introduce a miraculous PTFE waterproof breathable membrane that will safeguard your tissue culture.

The primary functions of the PTFE breathable membrane on culture media are: enabling gas exchange, preventing external contamination, and balancing internal and external gas pressures.

• The design of the breathable membrane allows for gas exchange, which is crucial for the normal physiological functions of cells. Cells require oxygen for respiration and must expel metabolic waste products like carbon dioxide. The breathable membrane provides an appropriate environment for gas exchange, maintaining normal metabolic activity in cell cultures.
• Moreover, the breathable membrane acts as an effective barrier, protecting the cell culture from external contaminants, keeping the interior environment clean, and reducing the risk of cross-contamination by bacteria
• Finally, the breathable membrane helps maintain gas pressure balance within cell shake flasks. During shake flask cultivation, the membrane can alleviate the accumulation of bubbles and pressure in the culture medium, avoiding stress concentration or abnormal pressure impacts on the cells.

In summary, the role of the breathable membrane on culture media lids is multifaceted.

XINNA's ePTFE back-adhesive venting membrane

XINNA recommends the PTFE breathable membrane, which is also known as the PTFE bacterial filtration membrane or PTFE sterile sealing breathable membrane. It is made from PTFE polymer through a special process into a breathable sealing membrane with properties of bacteria isolation and hydrophobicity. It can vent and depressurize effectively, preventing bacterial entry while ensuring an adequate supply of oxygen, thereby promoting the healthy growth of cultures. With high air permeability, heat resistance, and suitability for various high-temperature sterilization processes, it ensures stability and reliability during microbial culture, guaranteeing the quality and safety of the cultures.

Specifications:

Advantages:

• The uniform distribution of micro-pores allows for waterproofing and breathability while effectively blocking dust.
• It has good air permeability, allowing for sealed filtration while still enabling the free flow of air.
• With superior hydrophobic properties, it can quickly restore breathability after contact with water, oil, and other liquids, making it suitable for sealing and sterilizing various types of tissue culture bottles.
• It can balance pressure, preventing air and moisture around the sealed components from entering the system, thereby protecting the integrity of the seal.
• It is resistant to ultraviolet rays and has strong chemical inertness, withstanding high temperatures up to 135°C. The membrane can be repeatedly sterilized at high temperatures and used for gas filtration.
• It has strong corrosion resistance, maintaining its filtering and sterilizing performance in various harsh environments, keeping sensitive equipment parts safely exposed outdoors.
• Available in multiple specifications to meet the application needs of different scenarios.

Applications :

• Culture media cap
• Culture vessel
• Cell shaker flask
• Edible fungi spore bag