‌Comprehensive Guide to Laboratory Autoclave (High-Pressure Steam Sterilizer)‌

‌I. Principles and Functions‌

Autoclaves use high-temperature, high-pressure saturated steam (typically 121°C, 0.1MPa) to denature microbial proteins, achieving sterilization‌This method is suitable for heat-resistant materials such as culture media, glassware, and surgical instruments, effectively eliminating all microorganisms, including bacterial spores‌

‌II. Classification and Structure‌

1. ‌Types‌

   • ‌Portable‌: Compact (8L–30L), lightweight, ideal for routine laboratory use‌
   • ‌Vertical‌: Larger capacity (30L–200L), designed for hospitals or bulk sterilization‌
   • ‌Pre-vacuum‌: Removes air before sterilization to enhance steam penetration, suitable for porous or complex items‌

2. ‌Key Components‌

   • Sealed chamber, pressure gauge, safety valve, exhaust valve, and heating elements‌
   • Advanced models feature automated control systems (e.g., microprocessor-regulated pressure/temperature/time)‌

‌III. Operating Procedures‌

1. ‌Preparation‌

   • Verify water level, safety valve integrity, and chamber sealing‌
   • Load items into the inner basket without overcrowding to ensure steam circulation‌

2. ‌Sterilization Phase‌

   • Heat to generate steam, expel residual air via exhaust valve, then close the valve‌
   • Maintain target pressure (e.g., 0.1MPa) for 15–30 minutes (adjust based on material type)‌

3. ‌Completion‌

   • Allow natural cooling until pressure drops to zero. Open the exhaust valve and lid cautiously to avoid burns‌

‌IV. Safety and Maintenance‌

1. ‌Safety Protocols‌

   • Operator training is mandatory. ‌Never open the lid before the pressure has fully released‌‌
   • For liquid sterilization, vent pressure gradually to prevent boiling overflow‌

2. ‌Maintenance‌

   • Regularly inspect safety valves, seals, and exhaust lines for blockages or wear‌
   • Remove mineral deposits post-use to maintain heating efficiency‌

‌V. Typical Applications‌

• ‌Laboratories‌: Sterilizing culture media, lab tools‌
• ‌Medical Settings‌: Disinfecting surgical instruments, dressings‌
• ‌Food Industry‌: Packaging and canned goods sterilization‌