500ml Grace's Insect Medium Wyatt's Supplementary Insect Cell Medium

Grace's Insect Medium & Supplementary Insect Cell Medium Grace's Modified Wyatt's Medium

product description:

Grace's insect medium is Grace's modified Wyatt medium, which is more similar in chemical composition to insect hemolymph, and Grace successfully established the first continuous insect cell line using this medium.

Product Usage:

Grace's Insect Medium was originally used for the cultivation of Australian White Star Orange Moth (Antherea eucalypti) cells. After appropriate supplementation, Grace's Insect Medium can be used to cultivate various insect cells, including a variety of Lepidoptera and some Diptera insects. cell.

This product contains amino acids, vitamins, inorganic salts, and other ingredients required for various types of cell culture, but does not contain proteins, lipids, or any growth factors, so this product should be used with serum or serum-free supplements.

Product ingredients:

L-glutamine (Glutamine)

It is an essential nutrient element in cell culture, but it is unstable in the solution and will spontaneously degrade. The medium without L-glutamine can adjust the amount of L-glutamine arbitrarily according to the research needs. Adding fresh L-glutamine or its substitutes is more conducive to cell growth

glucose

The concentration of glucose can be adjusted at will according to the needs of the research, which is convenient and quick. High-glucose medium is widely used in fast-growing, low-adhesion cells, myeloma cells of hybridomas, cloned cells, transformed cells by DNA transfection, various primary virus host cells, single cell culture, and vaccine production. Production, such as the use of CHO cells to express EPO and produce hepatitis B vaccine. A low glucose medium is more suitable for the culture of slower metabolism, dependent adherent cells.

Precautions:

This product has been sterilized by filtration. Care should be taken during an aseptic operation to avoid contamination.

Do not freeze or thaw the product to keep the product in optimal condition.

This product is for scientific research or further research use only, not for diagnosis or treatment.