Cell Culture Medium

Ham's F-10 Nutrient Mixture cell culture medium Ham's F-10 modified medium

product description:

Ham's F-10 Nutrient Mixture (Ham's F-10 Nutrient Mixture) is a medium designed by Ham in 1963 for the serum-free culture of CHO cells.

Product advantages:

Ham's F-10 contains rich nutrients and is suitable for the culture of human diploid cells, the cloning culture of cells under low serum conditions, the normal culture of mammalian cells, the chromosome analysis of white blood cells, and rats, rabbits, chickens, etc. Animal primary cell culture.

For a wide range of cell culture applications, we offer Ham’s F-10 Modified Medium in a variety of components.

Product ingredients:

L-glutamine (Glutamine) is an essential nutrient element in cell culture, but it is unstable in solution and will spontaneously degrade. The medium without L-glutamine can be adjusted arbitrarily according to research needs. - The amount of glutamine, adding fresh L-glutamine or its substitutes is more conducive to the growth of cells when using.

Phenol red is used as an indicator of pH value in the culture medium to continuously monitor the pH of the culture medium. At low pH values, phenol red makes the culture medium yellow, while at higher pH values, it makes the culture medium purple. , when the pH value is 7.2 to 7.4, it is red, which is most suitable for cell culture. However, phenol red also has some disadvantages. Therefore, it is best to use a medium without phenol red when using it. Phenol red interferes with detection during flow cytometry analysis. In addition, the presence of phenol red in some formulations of serum-free media interferes with sodium-potassium balance.

Precautions:

This product is sterilized by filtration, and aseptic operation should be paid attention to when using it to avoid contamination;

In order to maintain the best use effect of this product, do not freeze or thaw;

This product is only used for scientific research or further research, not for diagnosis and treatment.