Cell Culture Medium

MCDB medium for culturing human microvascular endothelial cells MCDB 131 medium for endothelial cells​

product description:

MCDB is a low-protein and serum-free culture medium designed for specific cells. MCDB 131 is a kind of MCDB medium, which was designed and developed by Knedler and Ham. It was originally used to culture human microvascular endothelial cells (HMVEC).

Product Usage:

In addition to endothelial cells, MCDB 131 medium can also be used for other cell types, such as hepatocytes, smooth muscle cells, cardiomyocytes, etc. MCDB 131 medium is different from the traditional medium in that it contains trace elements, putrescine, adenine, thymine, and higher concentrations of amino acids and vitamins, and it is often used together with EGF, hydrocortisone, glutamine, and Low-concentration serum is used together, and the concentration of serum added must be determined according to the cell type.

For a wide range of cell culture applications, we offer MCDB-modified media in a variety of components.

Glucose can freely adjust the concentration of glucose according to research needs, which is convenient and quick. High-glucose medium is widely used in fast-growing, low-adhesion cells, myeloma cells of hybridomas, cloned cells, transformed cells by DNA transfection, various primary virus host cells, single cell culture, and vaccine production. Production, such as the use of CHO cells to express EPO and produce hepatitis B vaccine. A low glucose medium is more suitable for the culture of slower metabolism, dependent adherent cells.

Phenol red is used as an indicator of pH value in the culture medium to continuously monitor the pH of the culture medium. At low pH values, phenol red makes the culture medium yellow, while at higher pH values, it makes the culture medium purple. , when the pH value is 7.2 to 7.4, it is red, which is most suitable for cell culture. However, phenol red also has some disadvantages. Therefore, it is best to use a medium without phenol red when using it. Phenol red interferes with detection during flow cytometry analysis. In addition, the presence of phenol red in some formulations of serum-free media interferes with sodium-potassium balance.

This product contains amino acids, vitamins, inorganic salts, and other ingredients required for various types of cell culture, but does not contain proteins, lipids, or any growth factors, so this product should be used with serum or serum-free supplements.

Precautions:

This product is sterilized by filtration, and aseptic operation should be paid attention to when using it to avoid contamination;

In order to maintain the best use effect of this product, do not freeze or thaw;

This product is only used for scientific research or further research, not for diagnosis and treatment.