RPMI-1640 cell culture medium

RPMI-1640 cell culture medium Improved McCoy's 5A medium laboratory cell culture medium

product description:

RPMI-1640 was developed by Moore et al. in 1967 at the Roswell Park Memorial Institute (RPMI) in Faro, New York, USA. RPMI is a type of cell culture medium developed by the Institute, 1640 is the culture code.

Product ingredients:

RPMI-1640 is a modified version of McCoy's 5A medium, using a bicarbonate buffer system, which is different from most mammalian cell culture mediums in its typical PH8 formulation.

Product Usage:

RPMI-1640 medium was originally specially designed for lymphocyte culture, and now it has been widely used in the culture of various normal cells and cancer cells, including HeLa cells, Jurkat cells, MCF-7 cells, PC12 cells, PBMC cells, astrocytes Glial cells and cancer cells, is one of the most widely used media.

Product advantages:

RPMI RPMI 1640 Medium is unique compared to other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle’s MEM and DMEM.

For a wide range of cell culture applications, we offer RPMI 1640 Modified Medium in various components.

L-glutamine (Glutamine) is an essential nutrient element in cell culture, but it is unstable in solution and will spontaneously degrade. The medium without L-glutamine can be adjusted arbitrarily according to research needs. - The amount of glutamine, adding fresh L-glutamine or its substitutes is more conducive to the growth of cells when using.

Glucose can freely adjust the concentration of glucose according to research needs, which is convenient and quick. High-glucose medium is widely used in fast-growing, low-adhesion cells, myeloma cells of hybridomas, cloned cells, transformed cells by DNA transfection, various primary virus host cells, single cell culture, and vaccine production. Production, such as the use of CHO cells to express EPO and produce hepatitis B vaccine. A low glucose medium is more suitable for the culture of slower metabolism, dependent adherent cells.