Factory Supply Cell Culture Plate Disposable Plastic Cell Culture Plate Flat Bottom Multi-Well Plate

Classification and selection of cell culture plates. Depending on the shape of the bottom, a distinction can be made between plates with flat bottoms and plates with round bottoms (type U and V cell culture plates). Flat bottom cells can be used for either type, but if the number of cells is small, such as in cloning experiments, you must use a 96-well flat bottom plate. In addition, flat-bottomed plates are usually used in experiments such as MTT, regardless of adherent or suspension cells. U-shaped plates or V-shaped plates are generally used only when certain special requirements are needed. For example, in immunology, when two different lymphocytes are mixed and cultured, they must be in contact with each other for stimulation. Therefore, a U-shaped plate is usually required, and the cells will collect in a small area due to gravity.

When using V-shaped plates, the area is smaller than this.

In general, when used in cell-killing experiments, V-shaped plates are often used to make the target cells in close contact, but this test can also be replaced by U-shaped plates (after adding cells, low-speed centrifugal), when it comes to growing cells, usually choose a flat bottom, and pay special attention to the material, it seems that the round bottom has never been used to grow cells. The round bottom is usually used for analysis, chemical reaction, or sample storage because the round bottom can easily absorb the liquid, while a flat bottom is not so easy to absorb the liquid. However, if you want to measure the absorbance, you need to buy a flat bottom. For most cell cultures, flat-bottomed culture plates are used because they are good for observation under a microscope, have a clear bottom, and the liquid level in the cell culture is relatively uniform; they are also good for MTT detection. Selection of culture plates.

There are 6, 12, 24, 48, 96, and 384 holes in Ji'an biological breeding wells. Ji'an Biotechnology provides cell culture plates of various models and capacities for you to choose from.

The difference and selection of flat bottom and round bottom (U-shaped and V-shaped) culture plates.

Different forms of culture plates serve different purposes. Flat bottom plates are generally used for experiments such as MTT, whether it is adherent cells or suspension cells. Absorbance must be measured using flat-bottomed culture plates. U-shaped or V-shaped plates are generally used for some special requirements. For example, in immunology, when two different lymphocytes are mixed in culture, they must come into contact with each other in order to stimulate them. Currently, U-shaped plates are commonly used because cells are aggregated in a small area by gravity.

The round-bottom culture plate is also used for isotope incorporation experiments, where cells need to be collected with a cell harvester, such as "mixed lymphocyte culture" and so on. V-type plates are commonly used in cell killing and immunological hemagglutination experiments.

Round bottom culture plates are also used for isotope incorporation experiments that require harvesting cells with a cell harvester, eg. B. "Mixed Lymphocyte Culture", etc. V-plates are commonly used in cell killing and immune agglutination experiments. Usually use the well bottom area and recommended volume of the different culture plates.

The liquid level of the culture medium added to different wells should not be too deep, generally in the range of 2~3mm, and the appropriate amount of liquid added to each culture well can be calculated based on the bottom area. different wells. If the amount of liquid added is too much, it will affect the gas exchange (oxygen exchange), and it is easy to overflow and cause pollution during transportation. The specific cell density added can be flexibly controlled according to the purpose of the experiment.

Specification

Cell Culture Products Catalogue-Yong Yue.pdf

Detail pictures