This product is based on the purification method of high-binding magnetic particles. The sample is lysed and digested under the action of the binding solution, and the DNA/RNA is released into the digestion solution. After adding the magnetic particles and the binding solution, the DNA/RNA will be adsorbed on the surface of the magnetic particles, while impurities such as proteins will not be adsorbed and removed. The particles adsorbed DNA/RNA is washed with a washing solution to remove proteins and impurities, and finally, DNA/RNA is eluted by the eluent NFW/AVE.
Features
The Kit carries superparamagnetic nanoparticles which are effectively sure to nucleic acids and a protected and eco-friendly extraction system.
The magnetic beads are covered thru a special process, enabling sturdy binding with nucleic acids and convenient elution.
The experimental manner is easy and efficient:
1) Following pretreatment, add a pattern to a lysis-binding buffer to allow phone lysis, RNA launch, and RNA binding to magnetic beads in one step;
2) Apply magnetic pressure enabling handy wash of the beads with buffers;
3) Elution of RNA from the beads with the use of water or TE buffer. The extraction manner is completely well-matched with automation.
Purified RNA can be at once used in a range of downstream experiments, which include PCR, gene sequencing library construction, etc.
Applicable instruments
96-channel nucleic acid extraction instruments, such as Magix 96, Swift 96, iTrack 96, Kingfisher Flex, EXM6000, BG-Abort-96, YN-AP96, Auto-Pure96, and the similar principle of magnetic bar method nucleic acid extraction instruments.
32/48-channel nucleic acid extractors, such as MagMix32, Smart32, EXM3000, BG-Abort-48, and magnetic nucleic acid extractors with similar principles and operating methods.
Specification
Nucleic acid extraction reagents.pdf
Result of COVID-19 samples extraction by Yongyue kits | |||||
da mix | bj mix |
Average |
Agent |
samples | |
ct value | Average | ct value | |||
30.6967 |
32.63617 | 31.6299 |
33.85163 |
Yongyue |
Medium dilution 10 times |
31.9515 | 33.2403 | ||||
35.2603 | 36.6847 | ||||
32.5271 |
34.03243 | 32.6389 |
33.9073 |
zy | |
32.8807 | 33.9707 | ||||
36.6895 | 35.1123 | ||||
30.7953 |
32.50197 | 31.4687 |
32.9758 |
Yongyue | |
31.8571 | 32.9581 | ||||
34.8535 | 34.5006 | ||||
28.2534 |
29.35197 | 28.3752 |
29.17943 |
zy |
Medium |
28.4188 | 29.3623 | ||||
31.3837 | 29.8008 | ||||
26.3697 |
27.85903 | 28.0096 |
29.17203 |
Yongyue | |
26.6810 | 28.418 | ||||
30.5264 | 31.0885 | ||||
26.3812 |
28.0336 | 27.518 |
28.98737 |
Yongyue | |
27.3404 | 28.3675 | ||||
30.3792 | 31.0766 |
KF nucleic acid extractor | zy nucleic acid extractor | tl nucleic acid extractor | |||
ct value | Average | ct value | Average | ct value | Average |
30.35 |
30.36 | 31.74 |
30.68 | 30.01 |
30.6 |
29.77 | 30.33 | 30.23 | |||
30.03 | 30.25 | 30.18 | |||
30.21 | 30.28 | 30.75 | |||
30.09 | 30.30 | 31.3 | |||
30.91 | 30.91 | 31.13 | |||
30.56 | 31.16 | 30.5 | |||
30.92 | 30.50 | 30.64 |
Picture details