Specifications
Brand Name :
BRED
Model Number :
BRED-029
Certification :
CE, ISO13485
Place of Origin :
China
MOQ :
1 kits
Price :
Negotiable
Payment Terms :
T/T,
Supply Ability :
10000 kits per year
Delivery Time :
3 - 5 working days
Packaging Details :
20T/Kit
Shelf Life :
12 months
Storage Temperature :
2 - 8 ℃
Description

Sperm DNA Fragmentation Sperm Chromatin Structure Assay Kit AOT

Flow Cell Methodology

Packing specification:

  • 20 tests/kit

Intended use:

  • The kit is designed to evaluate the DNA integrity and maturity of human sperm by staining sperm chromatin.

Test principle:

After denatured by acid, fragmented sperm chromatin would turn to single strand. And the normal sperm chromatin retain double strand. Acridine Orange(AO) associated with single-stranded DNA emits red or yellow fluorescence at 488nm luminescence. While AO intercalated in double stranded DNA emits green fluorescene. If the red fluorescence is increasing, it shows a high rate of DNA fragmentation in sperms.

Main compositions:

  • Reagent A 1 vial 2.5 ml
  • Reagent B 1 vial 4.5 ml
  • Reagent C ( lyophilized powder ) 2 vials 13 ml 6.5ml/vial
  • Lyophilized powder dissolved solution 1 vial 4 ml

Storage conditions and duration:

  • Storage conditions: The kit is kept at 2 ~ 8 ℃ in dark place.
  • Period of validity: The kit is kept at 2 ~ 8 ℃ for one year. After dissolved, the reagent C would be kept for two weeks in dark at 2 ~ 8 ℃. Once separately store the dissolved reagent C at -20 ℃ in dark, the use life of reagent C could be more than one year.

Applicable instrument:

  • Flow cytometer (488nm luminescence)

Specimen requirements:

  • The sample should be collected after a minimum of 48 hours but not longer than seven days of sexual abstinency by masturbation or sexual intercourse using special condoms. The semen sample must be complete in collection.
  • Fresh semen samples should be liquefied completely prior to testing. If the samples have delayed liquefaction or high viscosity, a liquefier (sold separately) should be conducted.

Kit to Evaluate Sperm DNA Integrity

Kit to Evaluate Sperm DNA Integrity

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Kit to Evaluate Sperm DNA Integrity

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Brand Name :
BRED
Model Number :
BRED-029
Certification :
CE, ISO13485
Place of Origin :
China
MOQ :
1 kits
Price :
Negotiable
Contact Supplier
Kit to Evaluate Sperm DNA Integrity
Kit to Evaluate Sperm DNA Integrity
Kit to Evaluate Sperm DNA Integrity

BRED Life Science Technology Inc.

Verified Supplier
7 Years
guangdong, shenzhen
Since 2009
Business Type :
Manufacturer, Distributor/Wholesaler, Exporter, Seller
Total Annual :
10000000-50000000
Employee Number :
100~150
Certification Level :
Verified Supplier
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