Clinical applications:
1. To evaluate the morphological characteristics of human sperm;
2. The normal reference value recommended by the World Health Organization (WHO): normal sperm >4%.
Principles of test:
1. Oxaanthracene in the dye solution is an acid dye and thioazabenzene is an alkaline dye. The two dyes can
make sperm show different colors after selective adsorption with sperm protein;
2. The basic proteins in sperm cytoplasm were peach red in combination with oxaanthracene; The acid protein
is fuchsia in combination with thioazabenzene.
Features:
Test effect:
Results: the acrosomal region of spermatozoa was light purple, the non acrosomal region was dark purple, and the tail was light red.