Purified Anti-Phencyclidine Mouse Monoclonal Antibody Drug of Abuse

Background:

Phencyclidine, also known as angel dust and myriad other street names, is a recreational, dissociative drug formerly used as an anesthetic agent, exhibiting hallucinogenic and neurotoxic effects. Developed in Germany in 1926, it was first patented in 1952 by the Parke-Davis pharmaceutical company and marketed under the brand name Sernyl. In chemical structure, PCP is an arylcyclohexylamine derivative, and, in pharmacology, it is a member of the family of dissociative an-esthetics. PCP works primarily as an NMDA receptor antagonist, which blocks the activity of the NMDA receptor and, like most antiglutamatergic hallucinogens, is significantly more dangerous than other categories of hallucinogens. Other NMDA receptor antagonists include ketamine, tiletamine, dextromethorphan and nitrous oxide. Although the primary psychoactive effects of the drug lasts for a few hours, the total elimination rate from the body typically extends eight days or longer.As a recreational drug, PCP may be ingested, smoked, or snorted. Phencyclidine modulates secretogranin II[sg II] ex-pression in prefrontal cortex tissue in the absence of afferent inputs. The nature of these changes is dependent upon the duration of exposure to and/or withdrawal from phencyclidine. It is used as an anesthetic by veterinarians and is illicitly taken for its hallucinogen effects.

Specification:

Description: Monoclonal Antibody to Phencyclindine (PCP)

Specificity: This antibody reacts with PCP.

Host Animal: Hybridization of P3x63-Ag 9.653 myeloma cells with spleen cells from

Balb/c mouse

Isotype: IgG1, kappa light chain

Source: Mouse ascites

Immunogen: PCP-BSA

Format: Purified, liquid

Purification: DEAE chromatography

Concentration: 3.43 mg/mL (OD280 nm, E^0.1% = 1.40)

Affinity Constant: Not determined

Buffer: 0.015M potassium phosphate buffer, 0.85% NaCl, 0.05% NaN₃ pH=7.2

Pairing Assays: N/A

Purity: >95% by HPLC and SDS-PAGE

Application: PCP quantitative assays by EIA

Storage: 2-8^o C for 1 year; -20⁰ C for long term