Recombinant 2019-nCoV Mouse Monoclonal Antibody Spike RBD Protein

Background

The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate. This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cellsobtained from a mouse immunized with purified, Recombinant SARS-CoV-2 (2019-nCoV) Spike RBD-mFc Protein. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

Specification Data

The neutralization activity is Measured by microneutralization assay in vitro. The virus microneutralizaiton (MN) test was performed on 293T-ACE2 cells infected with SARS-CoV-2 (2019-nCoV) Spike Pseudovirus under treatment of serial dilutions of neutralizing antibody. The infection was neutralized by increasing concentrations of Anti-SARS-CoV-2 Neutralizing Antibody (Catalog# 40592-MM57). Rate of inhibition was determined by comparing the Relative Light Unit (RLU) of Luciferase reporter in different antibody concentrations. The IC50 is typically 0.41 µg/mL.

Loaded SARS-CoV-2 (2019-nCoV) Spike RBD-His Recombinant Protein,Biotinylated on SA Biosensor, can bind SARS-CoV-2 (2019-nCoV) Spike Neutralizing Antibody, Mouse MAb with an affinity constant of 0.07 nM as determined by Octet RED System.

Loaded SARS-CoV-2 (2019-nCoV) Spike S1-His Recombinant Protein,Biotinylated on SA Biosensor, can bind SARS-CoV-2 (2019-nCoV) Spike Neutralizing Antibody, Mouse MAb with an affinity constant of 0.05 nM as determined by Octet RED System

FAQ

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A: We are a factory, we have rich experience for manufacturing Medical Device more than 10 years.
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A: Our factory is located in Jiaxing city, Zhejiang Province, China.
It is about more than 1 hours from Shanghai.

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A: If any damaged rings in the course of carriage, with the checking it's our mistake, we will take full responsibility to compensate the customers.

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