Description:
The synthesizer is an economical, versatile mid-throughput platform with short cycle times and low reagent consumption. The system is ideal for the synthesis of primers of various lengths, as well as complex modified oligonucleotides and dye-labeled primers and probes. It can flexibly synthesize sequences longer than 120 mer at 25nmol-300umol synthesis scales. Equipped with 12 channels, each channel can be independently controlled to run, and different reaction times can be set individually to ensure the synthesis quality of each primer while synthesizing 12 primers in parallel.
DNA/RNA oligonucleotides produced by Honya Biotech can be used in gene synthesis, PCR/RT-PCR, siRNA/RNAi, DNA/RNA sequencing, immunology and biochemistry, high-throughput screening, natural product biosynthesis, gene construction, inversion Record PCR, hybridization, single nucleotide polymorphism detection, gene diagnosis and treatment research.
Technical Spots:
1 |
lightweight and modular design |
which is designed easy to add or removing modules. And optimize the configuration in future. |
2 |
Compact design |
which using an embedded control computer, no more space for computer, shortening half of the length than other similar synthesizer |
3 |
Graphical operation mode |
In the testing phase and the initial stage of synthesis, the pipeline can be tested automatically, the status of liquids, pipelines, valves, etc. |
4 |
Larger synthesis scale |
dual-axis motion system reduced the length of the column support plate ,also saves a lot of space to put larger scale columns. And it can synthesize 12 primers at the same time, which is twice as large as the traditional synthesizer |
5 |
AMI/MODIFY can be flexibly configured |
Base amidite and modified amidite can be set freely, and you can choose different Synthesis programme |
6 |
OXI/SUL can be configured separately |
OXI and SUL can be configured separately. |
7 |
Independent programme of each channel |
Each channel can select different sequence files and process parameters, which can set the initial synthesis position of the sequence and Initialization/finalization mode respectively, etc. For each channels programme can be saved, loaded, tested, and statistically analyzed. |
8 |
Real-time display of the synthesis process |
During the synthesis process, it can be paused manually or automatically, and then the operate the amidite and other reagent. |
Specification:
Channel |
12 |
Synthesis type |
DNA/RNA.fluorescent dyes/sulfurizing/Probe |
Mode |
reagent injection separately |
period(20 mer) |
12 primers of 20bp with 2.5h |
Cycled Time |
4-6min |
synthesis range |
25nmol-300umol |
Coupling rate |
>99% |
max length of primer |
120bp |
Bearing method |
Synthetic column |
wastes |
vacuum |
base/reagent pouring mode |
reagent injection separately |
base/reagent drive way |
protected gas press out |
Base cylinder quantities |
Standard 12 pcs,20pcs optional |
reagent bottle quantities |
7 pcs |
reagent cylinder configuration |
single standard. double optional |
reagent specifications |
450ml/4L |
synthesis merger base |
Automatic merge the base |
System |
Win7 |
Data import method |
Automatic by Excel |
reagent generality |
general |
Gas needed |
Nitrogen or Argon |
color |
Matte Black |
reagent pipe dimension |
OD1/8' and ID/16' |
base pipe dimension |
OD1/16, ID0.8mm |
outsize |
910*650*540mm |
package size |
1200*700*600mm |
1. Dual Axis motion system:
For traditional synthesis pedestal. There’s one line includes 12 base with long motion length and more space. We updated this technology divided one line into 2 lines. Which saves the synthesis motion time, and saves more space in the box to decrease the synthesis time efficiently.
2. Independent Pipeline System:
For each pipeline of amidite and reagents. We have the independent pipe to avoid pollution interaction between the reagents, the advantage is there’s no dead volume. It also can save the time and cost to clean the pipes by ACN. And each channel can choose the different sequence file and parameter and set Initialization/finalization mode freely. The programme for each pipes can be stored, loaded, inspected and make analysis.