1.2ml freezing Ampoul packaged equality control bacterial for test sterility
Quality Control Strains
Training method
Medium 1 modified Martin medium: peptone 5.0 g, yeast extract 2.0 g, glucose 20.0 g, dipotassium hydrogen phosphate 1.0 g, magnesium sulfate 0.5 g, agar 20.0 g, distilled water 1.0 L. pH 6.4 ± 0.2 at 121 ° C for 15 minutes. 2 or integrated PDA medium: 1000 mL of potato boiled juice, 3.0 g of potassium dihydrogen phosphate, 1.5 g of magnesium sulfate heptahydrate, 20.0 g of glucose, 10 mg of vitamin B1, 20 g of agar, and a natural pH. Potato boiled liquid: 200g potato, peeled, diced, boiled in distilled water for 30min, take the filtrate to a volume of 1000mL, and set aside.
Growth conditions
25-28 ° C, strict aerobic
Growth characteristics
The colony is white and filamentous, producing a large number of black spores black, the reverse side is yellowish brown, the surface of the colony is flat, there are radial grooves, the texture is velvety, and there is a small amount of colorless exudate. Conidial head ball type, diameter 50-80μm; spore stem diameter 10-15μm, smooth wall; apical spheroid, diameter 30-40μm, surface fully fertile; sporulation double layer, stalk base 10-12× 2-3 μm; bottle stem 6-8 × 2-3 μm; conidial sphere type, diameter 3-4 μm, rough wall.
Storage condition
4 ° C, protected from light and cold
Bacterial name | Bacterial number |
Pseudomonas aeruginosa | CMCC(B)10104 |
Staphylococcus aureus | CMCC(B)26003 |
Bacillus subtilis | CMCC(B)63501 |
Candida albicans | CMCC(F)98001 |
Aspergillus niger | CMCC(F)98003 |
Clostridium sporogenes | CMCC(B)64941 |
Escherichia coli | CMCC(B)44102 |
use of Quality Control Strains:
Sterility examination, microbiological limit inspection of non-sterile products, and bacteriostatic efficacy check.
Media suitability test, growth promotion test, sensitivity test, method suitability test, positive control, etc.